1mjh: Difference between revisions

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Revision as of 13:44, 20 March 2008

File:1mjh.gif

, resolution 1.7Å

Ligands:

and

Gene:

MJ0577 (Methanocaldococcus jannaschii)

Coordinates:

save as pdb, mmCIF, xml

STRUCTURE-BASED ASSIGNMENT OF THE BIOCHEMICAL FUNCTION OF HYPOTHETICAL PROTEIN MJ0577: A TEST CASE OF STRUCTURAL GENOMICS

OverviewOverview

Many small bacterial, archaebacterial, and eukaryotic genomes have been sequenced, and the larger eukaryotic genomes are predicted to be completely sequenced within the next decade. In all genomes sequenced to date, a large portion of these organisms' predicted protein coding regions encode polypeptides of unknown biochemical, biophysical, and/or cellular functions. Three-dimensional structures of these proteins may suggest biochemical or biophysical functions. Here we report the crystal structure of one such protein, MJ0577, from a hyperthermophile, Methanococcus jannaschii, at 1.7-A resolution. The structure contains a bound ATP, suggesting MJ0577 is an ATPase or an ATP-mediated molecular switch, which we confirm by biochemical experiments. Furthermore, the structure reveals different ATP binding motifs that are shared among many homologous hypothetical proteins in this family. This result indicates that structure-based assignment of molecular function is a viable approach for the large-scale biochemical assignment of proteins and for discovering new motifs, a basic premise of structural genomics.

About this StructureAbout this Structure

1MJH is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

ReferenceReference

Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics., Zarembinski TI, Hung LW, Mueller-Dieckmann HJ, Kim KK, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15189-93. PMID:9860944

Page seeded by OCA on Thu Mar 20 12:44:10 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1mjh.gif|left|200px]]<br /><applet load="1mjh" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1mjh.gif|left|200px]]
-caption="1mjh, resolution 1.7&Aring;" />
-'''STRUCTURE-BASED ASSIGNMENT OF THE BIOCHEMICAL FUNCTION OF HYPOTHETICAL PROTEIN MJ0577: A TEST CASE OF STRUCTURAL GENOMICS'''<br />
+ {{Structure
+|PDB= 1mjh |SIZE=350|CAPTION= <scene name='initialview01'>1mjh</scene>, resolution 1.7&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene>
+|ACTIVITY=
+|GENE= MJ0577 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
+}}
+'''STRUCTURE-BASED ASSIGNMENT OF THE BIOCHEMICAL FUNCTION OF HYPOTHETICAL PROTEIN MJ0577: A TEST CASE OF STRUCTURAL GENOMICS'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-MJH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJH OCA].
+MJH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJH OCA].
 ==Reference==
-Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics., Zarembinski TI, Hung LW, Mueller-Dieckmann HJ, Kim KK, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15189-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9860944 9860944]
+Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics., Zarembinski TI, Hung LW, Mueller-Dieckmann HJ, Kim KK, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15189-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9860944 9860944]
 [[Category: Methanocaldococcus jannaschii]]
 [[Category: Single protein]]
@@ Line 30: / Line 39: @@
 [[Category: protein structure initiative]]
 [[Category: psi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:47 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:44:10 2008''