1mi7: Difference between revisions

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Revision as of 13:43, 20 March 2008

File:1mi7.gif

, resolution 2.50Å

Ligands:

Gene:

trpR (Escherichia coli)

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol

OverviewOverview

The E. coli trp repressor (trpR) homodimer recognizes its palindromic DNA binding site through a pair of flexible helix-turn-helix (HTH) motifs displayed on an intertwined helical core. Flexible N-terminal arms mediate association between dimers bound to tandem DNA sites. The 2.5 A X-ray structure of trpR crystallized in 30% (v/v) isopropanol reveals a substantial conformational rearrangement of HTH motifs and N-terminal arms, with the protein appearing in the unusual form of an ordered 3D domain-swapped supramolecular array. Small angle X-ray scattering measurements show that the self-association properties of trpR in solution are fundamentally altered by isopropanol.

About this StructureAbout this Structure

1MI7 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

E. coli trp repressor forms a domain-swapped array in aqueous alcohol., Lawson CL, Benoff B, Berger T, Berman HM, Carey J, Structure. 2004 Jun;12(6):1099-108. PMID:15274929

Page seeded by OCA on Thu Mar 20 12:43:41 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1mi7.gif|left|200px]]<br /><applet load="1mi7" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1mi7.gif|left|200px]]
-caption="1mi7, resolution 2.50&Aring;" />
-'''Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol'''<br />
+ {{Structure
+|PDB= 1mi7 |SIZE=350|CAPTION= <scene name='initialview01'>1mi7</scene>, resolution 2.50&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=IPA:ISOPROPYL ALCOHOL'>IPA</scene>
+|ACTIVITY=
+|GENE= trpR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+}}
+'''Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-MI7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA].
+MI7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MI7 OCA].
 ==Reference==
-E. coli trp repressor forms a domain-swapped array in aqueous alcohol., Lawson CL, Benoff B, Berger T, Berman HM, Carey J, Structure. 2004 Jun;12(6):1099-108. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15274929 15274929]
+E. coli trp repressor forms a domain-swapped array in aqueous alcohol., Lawson CL, Benoff B, Berger T, Berman HM, Carey J, Structure. 2004 Jun;12(6):1099-108. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15274929 15274929]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: domain swapping]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:25 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:43:41 2008''