3u3o: Difference between revisions

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-[[Image:3u3o.png|left|200px]]
+==Crystal structure of Human SULT1A1 bound to PAP and two 3-Cyano-7-hydroxycoumarin==
+<StructureSection load='3u3o' size='340' side='right' caption='[[3u3o]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3u3o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U3O FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3QV:7-HYDROXY-2-OXO-2H-CHROMENE-3-CARBONITRILE'>3QV</scene>, <scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3u3j|3u3j]], [[3u3k|3u3k]], [[3u3m|3u3m]], [[3u3r|3u3r]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hSULT1A1, OK/SW-cl.88, STP, STP1, SULT1A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u3o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u3o RCSB], [http://www.ebi.ac.uk/pdbsum/3u3o PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cytosolic sulfotransferases (SULTs) are mammalian enzymes that detoxify a wide variety of chemicals through the addition of a sulfate group. Despite extensive research, the molecular basis for the broad specificity of SULTs is still not understood. Here, structural, protein engineering and kinetic approaches were employed to obtain deep understanding of the molecular basis for the broad specificity, catalytic activity and substrate inhibition of SULT1A1. We have determined five new structures of SULT1A1 in complex with different acceptors, and utilized a directed evolution approach to generate SULT1A1 mutants with enhanced thermostability and increased catalytic activity. We found that active site plasticity enables binding of different acceptors and identified dramatic structural changes in the SULT1A1 active site leading to the binding of a second acceptor molecule in a conserved yet non-productive manner. Our combined approach highlights the dominant role of SULT1A1 structural flexibility in controlling the specificity and activity of this enzyme.
-{{STRUCTURE_3u3o|  PDB=3u3o  |  SCENE=  }}
+The molecular basis for the broad substrate specificity of human sulfotransferase 1A1.,Berger I, Guttman C, Amar D, Zarivach R, Aharoni A PLoS One. 2011;6(11):e26794. Epub 2011 Nov 1. PMID:22069470<ref>PMID:22069470</ref>
-===Crystal structure of Human SULT1A1 bound to PAP and two 3-Cyano-7-hydroxycoumarin===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_22069470}}
-==About this Structure==
-[[3u3o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U3O OCA].
 ==See Also==
 *[[Sulfotransferase|Sulfotransferase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:022069470</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Aryl sulfotransferase]]
 [[Category: Homo sapiens]]
-[[Category: Aharoni, A.]]
+[[Category: Aharoni, A]]
-[[Category: Berger, I.]]
+[[Category: Berger, I]]
-[[Category: Guttman, C.]]
+[[Category: Guttman, C]]
-[[Category: Zarivach, R.]]
+[[Category: Zarivach, R]]
 [[Category: 3-cyano-7-hydroxycoumarin]]
 [[Category: Arylsulfotransferase]]