1m4z: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1m4z.jpg|left|200px]] | [[Image:1m4z.jpg|left|200px]] | ||
'''Crystal structure of the N-terminal BAH domain of Orc1p''' | {{Structure | ||
|PDB= 1m4z |SIZE=350|CAPTION= <scene name='initialview01'>1m4z</scene>, resolution 2.2Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | |||
|ACTIVITY= | |||
|GENE= Orc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''Crystal structure of the N-terminal BAH domain of Orc1p''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1M4Z is a [ | 1M4Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M4Z OCA]. | ||
==Reference== | ==Reference== | ||
Structure and function of the BAH-containing domain of Orc1p in epigenetic silencing., Zhang Z, Hayashi MK, Merkel O, Stillman B, Xu RM, EMBO J. 2002 Sep 2;21(17):4600-11. PMID:[http:// | Structure and function of the BAH-containing domain of Orc1p in epigenetic silencing., Zhang Z, Hayashi MK, Merkel O, Stillman B, Xu RM, EMBO J. 2002 Sep 2;21(17):4600-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198162 12198162] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: transcriptional silencing]] | [[Category: transcriptional silencing]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:38:56 2008'' | ||
Revision as of 13:38, 20 March 2008
| |||||||
| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | Orc1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the N-terminal BAH domain of Orc1p
OverviewOverview
The N-terminal domain of the largest subunit of the Saccharomyces cerevisiae origin recognition complex (Orc1p) functions in transcriptional silencing and contains a bromo-adjacent homology (BAH) domain found in some chromatin-associated proteins including Sir3p. The 2.2 A crystal structure of the N-terminal domain of Orc1p revealed a BAH core and a non-conserved helical sub-domain. Mutational analyses demonstrated that the helical sub-domain was necessary and sufficient to bind Sir1p, and critical for targeting Sir1p primarily to the cis-acting E silencers at the HMR and HML silent chromatin domains. In the absence of the BAH domain, approximately 14-20% of cells in a population were silenced at the HML locus. Moreover, the distributions of the Sir2p, Sir3p and Sir4p proteins, while normal, were at levels lower than found in wild-type cells. Thus, in the absence of the Orc1p BAH domain, HML resembled silencing of genes adjacent to telomeres. These data are consistent with the view that the Orc1p-Sir1p interaction at the E silencers ensures stable inheritance of pre-established Sir2p, Sir3p and Sir4p complexes at the silent mating type loci.
About this StructureAbout this Structure
1M4Z is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Structure and function of the BAH-containing domain of Orc1p in epigenetic silencing., Zhang Z, Hayashi MK, Merkel O, Stillman B, Xu RM, EMBO J. 2002 Sep 2;21(17):4600-11. PMID:12198162
Page seeded by OCA on Thu Mar 20 12:38:56 2008