1lwc: Difference between revisions

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Revision as of 13:36, 20 March 2008

File:1lwc.jpg

, resolution 2.62Å

Ligands:

and

Gene:

HIV-1 POL (Human immunodeficiency virus 1)

Activity:

RNA-directed DNA polymerase, with EC number 2.7.7.49

Coordinates:

save as pdb, mmCIF, xml

CRYSTAL STRUCTURE OF M184V MUTANT HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH NEVIRAPINE

OverviewOverview

Six structures of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) containing combinations of resistance mutations for zidovudine (AZT) (M41L and T215Y) or lamivudine (M184V) have been determined as inhibitor complexes. Minimal conformational changes in the polymerase or nonnucleoside RT inhibitor sites compared to the mutant RTMC (D67N, K70R, T215F, and K219N) are observed, indicating that such changes may occur only with certain combinations of mutations. Model building M41L and T215Y into HIV-1 RT-DNA and docking in ATP that is utilized in the pyrophosphorolysis reaction for AZT resistance indicates that some conformational rearrangement appears necessary in RT for ATP to interact simultaneously with the M41L and T215Y mutations.

About this StructureAbout this Structure

1LWC is a Protein complex structure of sequences from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of Zidovudine- or Lamivudine-resistant human immunodeficiency virus type 1 reverse transcriptases containing mutations at codons 41, 184, and 215., Chamberlain PP, Ren J, Nichols CE, Douglas L, Lennerstrand J, Larder BA, Stuart DI, Stammers DK, J Virol. 2002 Oct;76(19):10015-9. PMID:12208978

Page seeded by OCA on Thu Mar 20 12:36:00 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1lwc.jpg|left|200px]]<br /><applet load="1lwc" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lwc.jpg|left|200px]]
-caption="1lwc, resolution 2.62&Aring;" />
-'''CRYSTAL STRUCTURE OF M184V MUTANT HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH NEVIRAPINE'''<br />
+ {{Structure
+|PDB= 1lwc |SIZE=350|CAPTION= <scene name='initialview01'>1lwc</scene>, resolution 2.62&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=NVP:11-CYCLOPROPYL-5,11-DIHYDRO-4-METHYL-6H-DIPYRIDO[3,2-B:2',3'-E][1,4]DIAZEPIN-6-ONE'>NVP</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49]
+|GENE= HIV-1 POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1])
+}}
+'''CRYSTAL STRUCTURE OF M184V MUTANT HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH NEVIRAPINE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-LWC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=NVP:'>NVP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWC OCA].
+LWC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWC OCA].
 ==Reference==
-Crystal structures of Zidovudine- or Lamivudine-resistant human immunodeficiency virus type 1 reverse transcriptases containing mutations at codons 41, 184, and 215., Chamberlain PP, Ren J, Nichols CE, Douglas L, Lennerstrand J, Larder BA, Stuart DI, Stammers DK, J Virol. 2002 Oct;76(19):10015-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12208978 12208978]
+Crystal structures of Zidovudine- or Lamivudine-resistant human immunodeficiency virus type 1 reverse transcriptases containing mutations at codons 41, 184, and 215., Chamberlain PP, Ren J, Nichols CE, Douglas L, Lennerstrand J, Larder BA, Stuart DI, Stammers DK, J Virol. 2002 Oct;76(19):10015-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12208978 12208978]
 [[Category: Human immunodeficiency virus 1]]
 [[Category: Protein complex]]
@@ Line 23: / Line 32: @@
 [[Category: PO4]]
 [[Category: 3tc]]
-[[Category: aids]]
+[[Category: aid]]
 [[Category: azt]]
-[[Category: drug resistance mutations]]
+[[Category: drug resistance mutation]]
 [[Category: hiv-1 reverse transcriptase]]
 [[Category: nevirapine]]
 [[Category: nrti]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:05 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:36:00 2008''