1lpw: Difference between revisions

Revision as of 13:33, 20 March 2008

Coordinates:

Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine

OverviewOverview

Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.

About this StructureAbout this Structure

1LPW is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine., Newby MI, Greenbaum NL, Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583

Page seeded by OCA on Thu Mar 20 12:33:46 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1lpw.gif|left|200px]]<br /><applet load="1lpw" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lpw.gif|left|200px]]
-caption="1lpw" />
-'''Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine'''<br />
+ {{Structure
+|PDB= 1lpw |SIZE=350|CAPTION= <scene name='initialview01'>1lpw</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-LPW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LPW OCA].
+LPW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LPW OCA].
 ==Reference==
-Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine., Newby MI, Greenbaum NL, Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12426583 12426583]
+Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine., Newby MI, Greenbaum NL, Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12426583 12426583]
 [[Category: Protein complex]]
 [[Category: Saccharomyces cerevisiae]]
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 [[Category: u2 snrna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:47:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:33:46 2008''