1ll6: Difference between revisions
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[[Image:1ll6.gif|left|200px]] | [[Image:1ll6.gif|left|200px]] | ||
'''STRUCTURE OF THE D169N MUTANT OF C. IMMITIS CHITINASE 1''' | {{Structure | ||
|PDB= 1ll6 |SIZE=350|CAPTION= <scene name='initialview01'>1ll6</scene>, resolution 2.8Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] | |||
|GENE= CTS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5501 Coccidioides immitis]) | |||
}} | |||
'''STRUCTURE OF THE D169N MUTANT OF C. IMMITIS CHITINASE 1''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1LL6 is a [ | 1LL6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LL6 OCA]. | ||
==Reference== | ==Reference== | ||
The structure of an allosamidin complex with the Coccidioides immitis chitinase defines a role for a second acid residue in substrate-assisted mechanism., Bortone K, Monzingo AF, Ernst S, Robertus JD, J Mol Biol. 2002 Jul 5;320(2):293-302. PMID:[http:// | The structure of an allosamidin complex with the Coccidioides immitis chitinase defines a role for a second acid residue in substrate-assisted mechanism., Bortone K, Monzingo AF, Ernst S, Robertus JD, J Mol Biol. 2002 Jul 5;320(2):293-302. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12079386 12079386] | ||
[[Category: Chitinase]] | [[Category: Chitinase]] | ||
[[Category: Coccidioides immitis]] | [[Category: Coccidioides immitis]] | ||
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[[Category: beta-alpha barrel]] | [[Category: beta-alpha barrel]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:32:08 2008'' | ||
Revision as of 13:32, 20 March 2008
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| , resolution 2.8Å | |||||||
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| Gene: | CTS1 (Coccidioides immitis) | ||||||
| Activity: | Chitinase, with EC number 3.2.1.14 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE D169N MUTANT OF C. IMMITIS CHITINASE 1
OverviewOverview
Allosamidin is a known inhibitor of class 18 chitinases. We show that allosamidin is a competitive inhibitor of the fungal chitinase CiX1 from Coccidioides immitis, with a K(i) of 60 nM. We report the X-ray structure of the complex and show that upon inhibitor binding the side-chain of Asp169 rotates to form an ion pair with the oxazolinium cation. The mechanism of action is thought to involve protonation of the leaving group by Glu171 and substrate assistance by the sugar acetamido moiety to form an oxazoline-like intermediate. We converted both amino acid residues to the corresponding amide and found that each mutation effectively abolishes enzyme activity. X-ray structures show the mutant enzymes retain the basic wild-type structure and that the loss of mutant activity is due to their altered chemical properties. The high affinity of allosamidin, and its similarity to the putative reaction intermediate, suggests it is a transition state analog. This helps validate our contention that the role of Asp169 is to electrostatically stabilize the reaction transition state.
About this StructureAbout this Structure
1LL6 is a Single protein structure of sequence from Coccidioides immitis. Full crystallographic information is available from OCA.
ReferenceReference
The structure of an allosamidin complex with the Coccidioides immitis chitinase defines a role for a second acid residue in substrate-assisted mechanism., Bortone K, Monzingo AF, Ernst S, Robertus JD, J Mol Biol. 2002 Jul 5;320(2):293-302. PMID:12079386
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