3ps5: Difference between revisions

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-[[Image:3ps5.png|left|200px]]
+==Crystal structure of the full-length Human Protein Tyrosine Phosphatase SHP-1==
+<StructureSection load='3ps5' size='340' side='right' caption='[[3ps5]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3ps5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PS5 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PTPN6, HCP, PTP1C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ps5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ps5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ps5 RCSB], [http://www.ebi.ac.uk/pdbsum/3ps5 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+SHP-1 belongs to the family of non-receptor protein tyrosine phosphatases (PTPs) and generally acts as a negative regulator in a variety of cellular signaling pathways. Previously the crystal structures of the tail-truncated SHP-1 and SHP-2 revealed an autoinhibitory conformation. To understand the regulatory mechanism of SHP-1, we have determined the crystal structure of the full length SHP-1 at 3.1 A. Although the tail was disordered in current structure, the huge conformational rearrangement of the N-SH2 domain and the incorporation of sulfate ions into the ligand-binding site of each domain indicate that the SHP-1 is in the open conformation. The N-SH2 domain in current structure is shifted away from the active site of the PTP domain to the other side of the C-SH2 domain, resulting in exposure of the active site. Meanwhile, the C-SH2 domain is twisted anticlockwise by about 110 degrees . In addition, a set of new interactions between two SH2 domains and between the N-SH2 and the catalytic domains is identified, which could be responsible for the stabilization of SHP-1 in the open conformation. Based on the structural comparison, a model for the activation of SHP-1 is proposed. (c) 2011 Wiley-Liss, Inc.
-{{STRUCTURE_3ps5|  PDB=3ps5  |  SCENE=  }}
+Crystal structure of human protein tyrosine phosphatase SHP-1 in the open conformation.,Wang W, Liu L, Song X, Mo Y, Komma C, Bellamy HD, Zhao ZJ, Zhou GW J Cell Biochem. 2011 Apr 4. doi: 10.1002/jcb.23125. PMID:21465528<ref>PMID:21465528</ref>
-===Crystal structure of the full-length Human Protein Tyrosine Phosphatase SHP-1===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_21465528}}
-==About this Structure==
-[[3ps5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PS5 OCA].
 ==See Also==
 *[[Tyrosine phosphatase|Tyrosine phosphatase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021465528</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
 [[Category: Protein-tyrosine-phosphatase]]
-[[Category: Bellamy, H D.]]
+[[Category: Bellamy, H D]]
-[[Category: Komma, C.]]
+[[Category: Komma, C]]
-[[Category: Liu, L.]]
+[[Category: Liu, L]]
-[[Category: Mo, Y.]]
+[[Category: Mo, Y]]
-[[Category: Song, X.]]
+[[Category: Song, X]]
-[[Category: Wang, W.]]
+[[Category: Wang, W]]
-[[Category: Zhao, Z J.]]
+[[Category: Zhao, Z J]]
-[[Category: Zhou, G W.]]
+[[Category: Zhou, G W]]
 [[Category: Hydrolase]]
 [[Category: Phosphatase]]