1l5e: Difference between revisions

Revision as of 13:26, 20 March 2008

Coordinates:

The domain-swapped dimer of CV-N in solution

OverviewOverview

The structure of the potent HIV-inactivating protein cyanovirin-N was previously found by NMR to be a monomer in solution and a domain-swapped dimer by X-ray crystallography. Here we demonstrate that, in solution, CV-N can exist both in monomeric and in domain-swapped dimeric form. The dimer is a metastable, kinetically trapped structure at neutral pH and room temperature. Based on orientational NMR constraints, we show that the domain-swapped solution dimer is similar to structures in two different crystal forms, exhibiting solely a small reorientation around the hinge region. Mutation of the single proline residue in the hinge to glycine significantly stabilizes the protein in both its monomeric and dimeric forms. By contrast, mutation of the neighboring serine to proline results in an exclusively dimeric protein, caused by a drastic destabilization of the monomer.

About this StructureAbout this Structure

1L5E is a Single protein structure of sequence from Nostoc ellipsosporum. Full crystallographic information is available from OCA.

ReferenceReference

The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures., Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM, Structure. 2002 May;10(5):673-86. PMID:12015150

Page seeded by OCA on Thu Mar 20 12:26:39 2008

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OCA

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-[[Image:1l5e.jpg|left|200px]]<br /><applet load="1l5e" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1l5e.jpg|left|200px]]
-caption="1l5e" />
-'''The domain-swapped dimer of CV-N in solution'''<br />
+ {{Structure
+|PDB= 1l5e |SIZE=350|CAPTION= <scene name='initialview01'>1l5e</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''The domain-swapped dimer of CV-N in solution'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-L5E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5E OCA].
+L5E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5E OCA].
 ==Reference==
-The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures., Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM, Structure. 2002 May;10(5):673-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12015150 12015150]
+The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures., Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM, Structure. 2002 May;10(5):673-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12015150 12015150]
 [[Category: Nostoc ellipsosporum]]
 [[Category: Single protein]]
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 [[Category: x-ray]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:41:29 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:26:39 2008''