1l2k: Difference between revisions

← Older edit Newer edit →

Revision as of 13:25, 20 March 2008

File:1l2k.gif

, resolution 1.50Å

Ligands:

, , and

Coordinates:

save as pdb, mmCIF, xml

Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.

OverviewOverview

From the first days of protein neutron structure determination sperm whale myoglobin was an object under investigation [Nature 224 (1969) 143, J. Mol. Biol. 220 (1991) 381]. Nevertheless myoglobin is still of interest [Proc. Natl. Acad. Sci. USA 97 (2000) 3872]. The feasibility of the monochromatic neutron diffractometer BIX-3 at the JRR-3M reactor at the JAERI [J. Phys. Chem. Solids 60 (1999) 1623], to collect high-resolution diffraction data in a relatively short time stimulated us to repeat the structural determination of myoglobin. The structure of metmyoglobin has been determined up to a resolution of 1.5 A. The hydrogen atoms were replaced in part, by deuterium soaking the crystals for more than 10 years in D(2)O. A refinement of all atoms has been performed including the refinement of individual mean square displacements and occupancies of the exchangeable protons in backbone hydrogen bonds. A method is described to show clear negative scattering densities of the H atoms. Water molecules within the protein and on the molecule surface are shown. The exchangeability of H atoms is correlated with structural distribution and flexibility.

About this StructureAbout this Structure

1L2K is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.

ReferenceReference

Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:12062378

Page seeded by OCA on Thu Mar 20 12:25:31 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1l2k.gif|left|200px]]<br /><applet load="1l2k" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1l2k.gif|left|200px]]
-caption="1l2k, resolution 1.50&Aring;" />
-'''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''<br />
+ {{Structure
+|PDB= 1l2k |SIZE=350|CAPTION= <scene name='initialview01'>1l2k</scene>, resolution 1.50&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ND4:AMMONIUM+CATION+WITH+D'>ND4</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=DOD:DEUTERATED WATER'>DOD</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-L2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=ND4:'>ND4</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=DOD:'>DOD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2K OCA].
+L2K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2K OCA].
 ==Reference==
-Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12062378 12062378]
+Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12062378 12062378]
 [[Category: Physeter catodon]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: heme protein]]
 [[Category: hydration structure]]
-[[Category: hydrogen atoms]]
+[[Category: hydrogen atom]]
 [[Category: neutron structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:35 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:25:31 2008''