1kqf: Difference between revisions

← Older edit Newer edit →

Revision as of 13:20, 20 March 2008

File:1kqf.gif

, resolution 1.60Å

Ligands:

, , , and

Activity:

Formate dehydrogenase, with EC number 1.2.1.2

Coordinates:

save as pdb, mmCIF, xml

FORMATE DEHYDROGENASE N FROM E. COLI

OverviewOverview

The structure of the membrane protein formate dehydrogenase-N (Fdn-N), a major component of Escherichia coli nitrate respiration, has been determined at 1.6 angstroms. The structure demonstrates 11 redox centers, including molybdopterin-guanine dinucleotides, five [4Fe-4S] clusters, two heme b groups, and a menaquinone analog. These redox centers are aligned in a single chain, which extends almost 90 angstroms through the enzyme. The menaquinone reduction site associated with a possible proton pathway was also characterized. This structure provides critical insights into the proton motive force generation by redox loop, a common mechanism among a wide range of respiratory enzymes.

About this StructureAbout this Structure

1KQF is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis of proton motive force generation: structure of formate dehydrogenase-N., Jormakka M, Tornroth S, Byrne B, Iwata S, Science. 2002 Mar 8;295(5561):1863-8. PMID:11884747

Page seeded by OCA on Thu Mar 20 12:20:51 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1kqf.gif|left|200px]]<br /><applet load="1kqf" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1kqf.gif|left|200px]]
-caption="1kqf, resolution 1.60&Aring;" />
-'''FORMATE DEHYDROGENASE N FROM E. COLI'''<br />
+ {{Structure
+|PDB= 1kqf |SIZE=350|CAPTION= <scene name='initialview01'>1kqf</scene>, resolution 1.60&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=6MO:MOLYBDENUM(VI)+ION'>6MO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2]
+|GENE=
+}}
+'''FORMATE DEHYDROGENASE N FROM E. COLI'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-KQF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=6MO:'>6MO</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=MGD:'>MGD</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=CDL:'>CDL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQF OCA].
+KQF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQF OCA].
 ==Reference==
-Molecular basis of proton motive force generation: structure of formate dehydrogenase-N., Jormakka M, Tornroth S, Byrne B, Iwata S, Science. 2002 Mar 8;295(5561):1863-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11884747 11884747]
+Molecular basis of proton motive force generation: structure of formate dehydrogenase-N., Jormakka M, Tornroth S, Byrne B, Iwata S, Science. 2002 Mar 8;295(5561):1863-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11884747 11884747]
 [[Category: Escherichia coli]]
 [[Category: Formate dehydrogenase]]
@@ Line 35: / Line 44: @@
 [[Category: mpt]]
 [[Category: oxidoreductase]]
-[[Category: secys]]
+[[Category: secy]]
 [[Category: selenium]]
 [[Category: selenocysteine]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:50 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:20:51 2008''