1k8a: Difference between revisions
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[[Image:1k8a.gif|left|200px]] | [[Image:1k8a.gif|left|200px]] | ||
'''Co-crystal structure of Carbomycin A bound to the 50S ribosomal subunit of Haloarcula marismortui''' | {{Structure | ||
|PDB= 1k8a |SIZE=350|CAPTION= <scene name='initialview01'>1k8a</scene>, resolution 3.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CAI:CARBOMYCIN+A'>CAI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Co-crystal structure of Carbomycin A bound to the 50S ribosomal subunit of Haloarcula marismortui''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1K8A is a [ | 1K8A is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K8A OCA]. | ||
==Reference== | ==Reference== | ||
The structures of four macrolide antibiotics bound to the large ribosomal subunit., Hansen JL, Ippolito JA, Ban N, Nissen P, Moore PB, Steitz TA, Mol Cell. 2002 Jul;10(1):117-28. PMID:[http:// | The structures of four macrolide antibiotics bound to the large ribosomal subunit., Hansen JL, Ippolito JA, Ban N, Nissen P, Moore PB, Steitz TA, Mol Cell. 2002 Jul;10(1):117-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12150912 12150912] | ||
[[Category: Haloarcula marismortui]] | [[Category: Haloarcula marismortui]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: NA]] | [[Category: NA]] | ||
[[Category: antibiotic]] | [[Category: antibiotic]] | ||
[[Category: carbomycin | [[Category: carbomycin some]] | ||
[[Category: macrolide]] | [[Category: macrolide]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:13:53 2008'' | ||
Revision as of 13:13, 20 March 2008
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| , resolution 3.00Å | |||||||
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| Ligands: | , , , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Co-crystal structure of Carbomycin A bound to the 50S ribosomal subunit of Haloarcula marismortui
OverviewOverview
Crystal structures of the Haloarcula marismortui large ribosomal subunit complexed with the 16-membered macrolide antibiotics carbomycin A, spiramycin, and tylosin and a 15-membered macrolide, azithromycin, show that they bind in the polypeptide exit tunnel adjacent to the peptidyl transferase center. Their location suggests that they inhibit protein synthesis by blocking the egress of nascent polypeptides. The saccharide branch attached to C5 of the lactone rings extends toward the peptidyl transferase center, and the isobutyrate extension of the carbomycin A disaccharide overlaps the A-site. Unexpectedly, a reversible covalent bond forms between the ethylaldehyde substituent at the C6 position of the 16-membered macrolides and the N6 of A2103 (A2062, E. coli). Mutations in 23S rRNA that result in clinical resistance render the binding site less complementary to macrolides.
About this StructureAbout this Structure
1K8A is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.
ReferenceReference
The structures of four macrolide antibiotics bound to the large ribosomal subunit., Hansen JL, Ippolito JA, Ban N, Nissen P, Moore PB, Steitz TA, Mol Cell. 2002 Jul;10(1):117-28. PMID:12150912
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