1k1g: Difference between revisions

Revision as of 13:11, 20 March 2008

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STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1

OverviewOverview

During spliceosome assembly, splicing factor 1 (SF1) specifically recognizes the intron branch point sequence (BPS) UACUAAC in the pre-mRNA transcripts. We show that the KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for BPS binding. The 3' part of the BPS (UAAC), including the conserved branch point adenosine (underlined), is specifically recognized in a hydrophobic cleft formed by the Gly-Pro-Arg-Gly motif and the variable loop of the KH domain. The QUA2 region recognizes the 5' nucleotides of the BPS (ACU). The branch point adenosine acting as the nucleophile in the first biochemical step of splicing is deeply buried. BPS RNA recognition suggests how SF1 may facilitate subsequent formation of the prespliceosomal complex A.

About this StructureAbout this Structure

1K1G is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for recognition of the intron branch site RNA by splicing factor 1., Liu Z, Luyten I, Bottomley MJ, Messias AC, Houngninou-Molango S, Sprangers R, Zanier K, Kramer A, Sattler M, Science. 2001 Nov 2;294(5544):1098-102. PMID:11691992

Page seeded by OCA on Thu Mar 20 12:11:15 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1k1g.gif|left|200px]]<br /><applet load="1k1g" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1k1g.gif|left|200px]]
-caption="1k1g" />
-'''STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1'''<br />
+ {{Structure
+|PDB= 1k1g |SIZE=350|CAPTION= <scene name='initialview01'>1k1g</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-K1G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1G OCA].
+K1G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1G OCA].
 ==Reference==
-Structural basis for recognition of the intron branch site RNA by splicing factor 1., Liu Z, Luyten I, Bottomley MJ, Messias AC, Houngninou-Molango S, Sprangers R, Zanier K, Kramer A, Sattler M, Science. 2001 Nov 2;294(5544):1098-102. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11691992 11691992]
+Structural basis for recognition of the intron branch site RNA by splicing factor 1., Liu Z, Luyten I, Bottomley MJ, Messias AC, Houngninou-Molango S, Sprangers R, Zanier K, Kramer A, Sattler M, Science. 2001 Nov 2;294(5544):1098-102. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11691992 11691992]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
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 [[Category: qua2 homology]]
 [[Category: splicing]]
-[[Category: star proteins]]
+[[Category: star protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:04 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:11:15 2008''