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'''NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase''' | {{Structure | ||
|PDB= 1jwe |SIZE=350|CAPTION= <scene name='initialview01'>1jwe</scene> | |||
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|LIGAND= | |||
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'''NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JWE is a [ | 1JWE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWE OCA]. | ||
==Reference== | ==Reference== | ||
NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer., Weigelt J, Brown SE, Miles CS, Dixon NE, Otting G, Structure. 1999 Jun 15;7(6):681-90. PMID:[http:// | NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer., Weigelt J, Brown SE, Miles CS, Dixon NE, Otting G, Structure. 1999 Jun 15;7(6):681-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10404597 10404597] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: helicase]] | [[Category: helicase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:09:22 2008'' | ||
Revision as of 13:09, 20 March 2008
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NMR Structure of the N-Terminal Domain of E. Coli Dnab Helicase
OverviewOverview
BACKGROUND: DnaB is the primary replicative helicase in Escherichia coli. Native DnaB is a hexamer of identical subunits, each consisting of a larger C-terminal domain and a smaller N-terminal domain. Electron-microscopy data show hexamers with C6 or C3 symmetry, indicating large domain movements and reversible pairwise association. RESULTS: The three-dimensional structure of the N-terminal domain of E. coli DnaB was determined by nuclear magnetic resonance (NMR) spectroscopy. Structural similarity was found with the primary dimerisation domain of a topoisomerase, the gyrase A subunit from E. coli. A monomer-dimer equilibrium was observed for the isolated N-terminal domain of DnaB. A dimer model with C2 symmetry was derived from intermolecular nuclear Overhauser effects, which is consistent with all available NMR data. CONCLUSIONS: The monomer-dimer equilibrium observed for the N-terminal domain of DnaB is likely to be of functional significance for helicase activity, by participating in the switch between C6 and C3 symmetry of the helicase hexamer.
About this StructureAbout this Structure
1JWE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
NMR structure of the N-terminal domain of E. coli DnaB helicase: implications for structure rearrangements in the helicase hexamer., Weigelt J, Brown SE, Miles CS, Dixon NE, Otting G, Structure. 1999 Jun 15;7(6):681-90. PMID:10404597
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