1jqq: Difference between revisions

Revision as of 13:07, 20 March 2008

, resolution 2.65Å

Coordinates:

Crystal structure of Pex13p(301-386) SH3 domain

OverviewOverview

While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combined use of X-ray crystallography, NMR spectroscopy, and circular dichroism, we show that the two ligands bind in unrelated conformations to patches located at opposite surfaces of this SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions within the Pex13p-Pex5p interface specifically impair PTS1-dependent protein import into yeast peroxisomes.

About this StructureAbout this Structure

1JQQ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain., Douangamath A, Filipp FV, Klein AT, Barnett P, Zou P, Voorn-Brouwer T, Vega MC, Mayans OM, Sattler M, Distel B, Wilmanns M, Mol Cell. 2002 Nov;10(5):1007-17. PMID:12453410

Page seeded by OCA on Thu Mar 20 12:07:07 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1jqq.jpg|left|200px]]<br /><applet load="1jqq" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jqq.jpg|left|200px]]
-caption="1jqq, resolution 2.65&Aring;" />
-'''Crystal structure of Pex13p(301-386) SH3 domain'''<br />
+ {{Structure
+|PDB= 1jqq |SIZE=350|CAPTION= <scene name='initialview01'>1jqq</scene>, resolution 2.65&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of Pex13p(301-386) SH3 domain'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-JQQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQQ OCA].
+JQQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQQ OCA].
 ==Reference==
-Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain., Douangamath A, Filipp FV, Klein AT, Barnett P, Zou P, Voorn-Brouwer T, Vega MC, Mayans OM, Sattler M, Distel B, Wilmanns M, Mol Cell. 2002 Nov;10(5):1007-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12453410 12453410]
+Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain., Douangamath A, Filipp FV, Klein AT, Barnett P, Zou P, Voorn-Brouwer T, Vega MC, Mayans OM, Sattler M, Distel B, Wilmanns M, Mol Cell. 2002 Nov;10(5):1007-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12453410 12453410]
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
@@ Line 18: / Line 27: @@
 [[Category: Mayans, O.]]
 [[Category: Wilmanns, M.]]
-[[Category: compact beta-barrel of five anti-parrallel beta-strands]]
+[[Category: compact beta-barrel of five anti-parrallel beta-strand]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:40 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:07 2008''