1jl7: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1jl7.jpg|left|200px]] | [[Image:1jl7.jpg|left|200px]] | ||
'''Crystal Structure Of CN-Ligated Component III Glycera Dibranchiata Monomeric Hemoglobin''' | {{Structure | ||
|PDB= 1jl7 |SIZE=350|CAPTION= <scene name='initialview01'>1jl7</scene>, resolution 1.4Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal Structure Of CN-Ligated Component III Glycera Dibranchiata Monomeric Hemoglobin''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1JL7 is a [ | 1JL7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycera_dibranchiata Glycera dibranchiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL7 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of unligated and CN-ligated Glycera dibranchiata monomer ferric hemoglobin components III and IV., Park HJ, Yang C, Treff N, Satterlee JD, Kang C, Proteins. 2002 Oct 1;49(1):49-60. PMID:[http:// | Crystal structures of unligated and CN-ligated Glycera dibranchiata monomer ferric hemoglobin components III and IV., Park HJ, Yang C, Treff N, Satterlee JD, Kang C, Proteins. 2002 Oct 1;49(1):49-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12211015 12211015] | ||
[[Category: Glycera dibranchiata]] | [[Category: Glycera dibranchiata]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: monomer hemoglobin]] | [[Category: monomer hemoglobin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:04:58 2008'' | ||
Revision as of 13:04, 20 March 2008
| |||||||
| , resolution 1.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Of CN-Ligated Component III Glycera Dibranchiata Monomeric Hemoglobin
OverviewOverview
Erythrocytes of the marine annelid, Glycera dibranchiata, contain a mixture of monomeric and polymeric hemoglobins. There are three major monomer hemoglobin components, II, III, IV (also called GMH2, 3, and 4), that have been highly purified and well characterized. We have now crystallized GMH3 and GMH4 and determined their structures to 1.4-1.8 A resolution. The structures were determined for these two monomer hemoglobins in the oxidized (Fe3+, ferric, or met-) forms in both the unligated and cyanide-ligated states. This work differs from two published, refined structures of a Glycera dibranchiata monomer hemoglobin, which has a sequence that is substantially different from any bona fide major monomer hemoglobins (GMH2, 3, or 4). The high-resolution crystal structures (presented here) and the previous NMR structure of CO-ligated GMH4, provide a basis for interpreting structure/function details of the monomer hemoglobins. These details include: (1) the strong correlation between temperature factor and NMR dynamics for respective protein forms; (2) the unique nature of the HisE7Leu primary sequence substitutions in GMH3 and GMH4 and their impact on cyanide ion binding kinetics; (3) the LeuB10Phe difference between GMH3 and GMH4 and its impact on ligand binding; and (4) elucidation of changes in the structural details of the distal and proximal heme pockets upon cyanide binding.
About this StructureAbout this Structure
1JL7 is a Single protein structure of sequence from Glycera dibranchiata. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of unligated and CN-ligated Glycera dibranchiata monomer ferric hemoglobin components III and IV., Park HJ, Yang C, Treff N, Satterlee JD, Kang C, Proteins. 2002 Oct 1;49(1):49-60. PMID:12211015
Page seeded by OCA on Thu Mar 20 12:04:58 2008