1jfa: Difference between revisions
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[[Image:1jfa.jpg|left|200px]] | [[Image:1jfa.jpg|left|200px]] | ||
'''TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES''' | {{Structure | ||
|PDB= 1jfa |SIZE=350|CAPTION= <scene name='initialview01'>1jfa</scene>, resolution 2.5Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Trichodiene_synthase Trichodiene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.6 4.2.3.6] | |||
|GENE= TRI5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5514 Fusarium sporotrichioides]) | |||
}} | |||
'''TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JFA is a [ | 1JFA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JFA OCA]. | ||
==Reference== | ==Reference== | ||
Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade., Rynkiewicz MJ, Cane DE, Christianson DW, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13543-8. Epub 2001 Nov 6. PMID:[http:// | Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade., Rynkiewicz MJ, Cane DE, Christianson DW, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13543-8. Epub 2001 Nov 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11698643 11698643] | ||
[[Category: Fusarium sporotrichioides]] | [[Category: Fusarium sporotrichioides]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: terpenoid synthase fold]] | [[Category: terpenoid synthase fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:02:41 2008'' | ||
Revision as of 13:02, 20 March 2008
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| , resolution 2.5Å | |||||||
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| Ligands: | |||||||
| Gene: | TRI5 (Fusarium sporotrichioides) | ||||||
| Activity: | Trichodiene synthase, with EC number 4.2.3.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES
OverviewOverview
The x-ray crystal structure of recombinant trichodiene synthase from Fusarium sporotrichioides has been determined to 2.5-A resolution, both unliganded and complexed with inorganic pyrophosphate. This reaction product coordinates to three Mg(2+) ions near the mouth of the active site cleft. A comparison of the liganded and unliganded structures reveals a ligand-induced conformational change that closes the mouth of the active site cleft. Binding of the substrate farnesyl diphosphate similarly may trigger this conformational change, which would facilitate catalysis by protecting reactive carbocationic intermediates in the cyclization cascade. Trichodiene synthase also shares significant structural similarity with other sesquiterpene synthases despite a lack of significant sequence identity. This similarity indicates divergence from a common ancestor early in the evolution of terpene biosynthesis.
About this StructureAbout this Structure
1JFA is a Single protein structure of sequence from Fusarium sporotrichioides. Full crystallographic information is available from OCA.
ReferenceReference
Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade., Rynkiewicz MJ, Cane DE, Christianson DW, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13543-8. Epub 2001 Nov 6. PMID:11698643
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