1jal: Difference between revisions
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[[Image:1jal.gif|left|200px]] | [[Image:1jal.gif|left|200px]] | ||
'''YCHF PROTEIN (HI0393)''' | {{Structure | ||
|PDB= 1jal |SIZE=350|CAPTION= <scene name='initialview01'>1jal</scene>, resolution 2.40Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= YchF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae]) | |||
}} | |||
'''YCHF PROTEIN (HI0393)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JAL is a [ | 1JAL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAL OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid., Teplyakov A, Obmolova G, Chu SY, Toedt J, Eisenstein E, Howard AJ, Gilliland GL, J Bacteriol. 2003 Jul;185(14):4031-7. PMID:[http:// | Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid., Teplyakov A, Obmolova G, Chu SY, Toedt J, Eisenstein E, Howard AJ, Gilliland GL, J Bacteriol. 2003 Jul;185(14):4031-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12837776 12837776] | ||
[[Category: Haemophilus influenzae]] | [[Category: Haemophilus influenzae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: nucleotide-binding fold]] | [[Category: nucleotide-binding fold]] | ||
[[Category: s2f]] | [[Category: s2f]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
[[Category: structure 2 function project]] | [[Category: structure 2 function project]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:00:44 2008'' | ||
Revision as of 13:00, 20 March 2008
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| , resolution 2.40Å | |||||||
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| Gene: | YchF (Haemophilus influenzae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
YCHF PROTEIN (HI0393)
OverviewOverview
The bacterial protein encoded by the gene ychF is 1 of 11 universally conserved GTPases and the only one whose function is unknown. The crystal structure determination of YchF was sought to help with the functional assignment of the protein. The YchF protein from Haemophilus influenzae was cloned and expressed, and the crystal structure was determined at 2.4 A resolution. The polypeptide chain is folded into three domains. The N-terminal domain has a mononucleotide binding fold typical for the P-loop NTPases. An 80-residue domain next to it has a pronounced alpha-helical coiled coil. The C-terminal domain features a six-stranded half-barrel that curves around an alpha-helix. The crablike three-domain structure of YchF suggests the binding site for a double-stranded nucleic acid in the cleft between the domains. The structure of the putative GTP-binding site is consistent with the postulated guanine specificity of the protein. Fluorescence measurements have demonstrated the ability of YchF to bind a double-stranded nucleic acid and GTP. Taken together with other experimental data and genomic analysis, these results suggest that YchF may be part of a nucleoprotein complex and may function as a GTP-dependent translation factor.
About this StructureAbout this Structure
1JAL is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid., Teplyakov A, Obmolova G, Chu SY, Toedt J, Eisenstein E, Howard AJ, Gilliland GL, J Bacteriol. 2003 Jul;185(14):4031-7. PMID:12837776
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