1iu9: Difference between revisions
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[[Image:1iu9.jpg|left|200px]] | [[Image:1iu9.jpg|left|200px]] | ||
'''Crystal structure of the C-terminal domain of aspartate racemase from Pyrococcus horikoshii OT3''' | {{Structure | ||
|PDB= 1iu9 |SIZE=350|CAPTION= <scene name='initialview01'>1iu9</scene>, resolution 2.04Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Aspartate_racemase Aspartate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.13 5.1.1.13] | |||
|GENE= pPH0670E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii]) | |||
}} | |||
'''Crystal structure of the C-terminal domain of aspartate racemase from Pyrococcus horikoshii OT3''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1IU9 is a [ | 1IU9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IU9 OCA]. | ||
==Reference== | ==Reference== | ||
Structural insight into gene duplication, gene fusion and domain swapping in the evolution of PLP-independent amino acid racemases., Liu L, Iwata K, Yohda M, Miki K, FEBS Lett. 2002 Sep 25;528(1-3):114-8. PMID:[http:// | Structural insight into gene duplication, gene fusion and domain swapping in the evolution of PLP-independent amino acid racemases., Liu L, Iwata K, Yohda M, Miki K, FEBS Lett. 2002 Sep 25;528(1-3):114-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12297289 12297289] | ||
[[Category: Aspartate racemase]] | [[Category: Aspartate racemase]] | ||
[[Category: Pyrococcus horikoshii]] | [[Category: Pyrococcus horikoshii]] | ||
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[[Category: crystal structure]] | [[Category: crystal structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:55:01 2008'' | ||
Revision as of 12:55, 20 March 2008
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| , resolution 2.04Å | |||||||
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| Ligands: | |||||||
| Gene: | pPH0670E (Pyrococcus horikoshii) | ||||||
| Activity: | Aspartate racemase, with EC number 5.1.1.13 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the C-terminal domain of aspartate racemase from Pyrococcus horikoshii OT3
OverviewOverview
The X-ray crystal structure has revealed two similar alpha/beta domains of aspartate racemase (AspR) from Pyrococcus horikoshii OT3, and identified a pseudo mirror-symmetric distribution of the residues around its active site [Liu et al. (2002) J. Mol. Biol. 319, 479-489]. Structural homology and functional similarity between the two domains suggested that this enzyme evolved from an ancestral domain by gene duplication and gene fusion. We have expressed solely the C-terminal domain of this AspR and determined its three-dimensional structure by X-ray crystallography. The high structural stability of this domain supports the existence of the ancestral domain. In comparison with other amino acid racemases (AARs), we suggest that gene duplication and gene fusion are conventional ways in the evolution of pyridoxal 5'-phosphate-independent AARs.
About this StructureAbout this Structure
1IU9 is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.
ReferenceReference
Structural insight into gene duplication, gene fusion and domain swapping in the evolution of PLP-independent amino acid racemases., Liu L, Iwata K, Yohda M, Miki K, FEBS Lett. 2002 Sep 25;528(1-3):114-8. PMID:12297289
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