1iqw: Difference between revisions
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'''CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE MOUSE ANTI-HUMAN FAS ANTIBODY HFE7A''' | {{Structure | ||
|PDB= 1iqw |SIZE=350|CAPTION= <scene name='initialview01'>1iqw</scene>, resolution 2.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE MOUSE ANTI-HUMAN FAS ANTIBODY HFE7A''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1IQW is a [ | 1IQW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IQW OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the antigen-binding fragment of apoptosis-inducing mouse anti-human Fas monoclonal antibody HFE7A., Ito S, Takayama T, Hanzawa H, Ichikawa K, Ohsumi J, Serizawa N, Hata T, Haruyama H, J Biochem. 2002 Jan;131(1):137-43. PMID:[http:// | Crystal structure of the antigen-binding fragment of apoptosis-inducing mouse anti-human Fas monoclonal antibody HFE7A., Ito S, Takayama T, Hanzawa H, Ichikawa K, Ohsumi J, Serizawa N, Hata T, Haruyama H, J Biochem. 2002 Jan;131(1):137-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11754745 11754745] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Takayama, T.]] | [[Category: Takayama, T.]] | ||
[[Category: agonistic antibody]] | [[Category: agonistic antibody]] | ||
[[Category: | [[Category: anti_fa]] | ||
[[Category: apoptosis]] | [[Category: apoptosis]] | ||
[[Category: fab]] | [[Category: fab]] | ||
[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:53:46 2008'' | ||
Revision as of 12:53, 20 March 2008
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CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE MOUSE ANTI-HUMAN FAS ANTIBODY HFE7A
OverviewOverview
Binding of Fas ligand to Fas induces apoptosis. The Fas-Fas ligand system plays important roles in many biological processes, including the elimination of autoreactive lymphoid cells. The mouse anti-human Fas monoclonal antibody HFE7A (m-HFE7A), which induces apoptosis, has been humanized based on a structure predicted by homology modeling. A version of humanized HFE7A is currently under development for the treatment of autoimmune diseases such as rheumatoid arthritis. For a deeper understanding of the protein engineering aspect of antibody humanization, for which information on the three-dimensional structure is essential, we determined the crystal structure of the m-HFE7A antigen-binding fragment (Fab) by X-ray crystallography at 2.5 A resolution. The main-chain conformation of the five loops in the six complementarity-determining regions (CDRs) was correctly predicted with root-mean-square deviations of 0.30-1.04 A based on a comparison of the crystal structure with the predicted structure. The CDR-H3 conformation of the crystal structure, which was not classified as one of the canonical structures, was completely different from that of the predicted structure but adopted the conformation which followed the "H3-rules." The results of charge distribution analysis of the antigen-binding site suggest that electrostatic interactions may be important for its binding to Fas.
About this StructureAbout this Structure
1IQW is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the antigen-binding fragment of apoptosis-inducing mouse anti-human Fas monoclonal antibody HFE7A., Ito S, Takayama T, Hanzawa H, Ichikawa K, Ohsumi J, Serizawa N, Hata T, Haruyama H, J Biochem. 2002 Jan;131(1):137-43. PMID:11754745
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