1ig3: Difference between revisions
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'''Mouse Thiamin Pyrophosphokinase Complexed with Thiamin''' | {{Structure | ||
|PDB= 1ig3 |SIZE=350|CAPTION= <scene name='initialview01'>1ig3</scene>, resolution 1.90Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=VIB:3-(4-AMINO-2-METHYL-PYRIMIDIN-5-YLMETHYL)-5-(2-HYDROXY-ETHYL)-4-METHYL-THIAZOL-3-IUM'>VIB</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Thiamine_diphosphokinase Thiamine diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.2 2.7.6.2] | |||
|GENE= | |||
}} | |||
'''Mouse Thiamin Pyrophosphokinase Complexed with Thiamin''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1IG3 is a [ | 1IG3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG3 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of thiamin pyrophosphokinase., Timm DE, Liu J, Baker LJ, Harris RA, J Mol Biol. 2001 Jun 29;310(1):195-204. PMID:[http:// | Crystal structure of thiamin pyrophosphokinase., Timm DE, Liu J, Baker LJ, Harris RA, J Mol Biol. 2001 Jun 29;310(1):195-204. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11419946 11419946] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: beta barrel]] | [[Category: beta barrel]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:49:55 2008'' | ||
Revision as of 12:49, 20 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Thiamine diphosphokinase, with EC number 2.7.6.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Mouse Thiamin Pyrophosphokinase Complexed with Thiamin
OverviewOverview
Thiamin pyrophosphate (TPP) is a coenzyme derived from vitamin B1 (thiamin). TPP synthesis in eukaryotes requires thiamin pyrophosphokinase (TPK), which catalyzes the transfer of a pyrophosphate group from ATP to thiamin. TPP is essential for central metabolic processes, including the formation of acetyl CoA from glucose and the Krebs cycle. Deficiencies in human thiamin metabolism result in beriberi and Wernicke encephalopathy. The crystal structure of mouse TPK was determined by multiwavelength anomalous diffraction at 2.4 A resolution, and the structure of TPK complexed with thiamin has been refined at 1.9 A resolution. The TPK polypeptide folds as an alpha/beta-domain and a beta-sandwich domain, which share a central ten-stranded mixed beta-sheet. TPK subunits associate as a dimer, and thiamin is bound in the dimer interface. Despite lacking apparent sequence homology with other proteins, the alpha/beta-domain resembles the Rossman fold and is similar to other kinase structures, including another pyrophosphokinase and a thiamin biosynthetic enzyme. Comparison of mouse and yeast TPK structures reveals differences that could be exploited in developing species-specific inhibitors of potential use as antimicrobial agents.
About this StructureAbout this Structure
1IG3 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of thiamin pyrophosphokinase., Timm DE, Liu J, Baker LJ, Harris RA, J Mol Biol. 2001 Jun 29;310(1):195-204. PMID:11419946
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