1ibk: Difference between revisions

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Revision as of 12:48, 20 March 2008

File:1ibk.gif

, resolution 3.31Å

Ligands:

, and

Coordinates:

save as pdb, mmCIF, xml

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN

OverviewOverview

Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or "wobble," position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.

About this StructureAbout this Structure

1IBK is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Recognition of cognate transfer RNA by the 30S ribosomal subunit., Ogle JM, Brodersen DE, Clemons WM Jr, Tarry MJ, Carter AP, Ramakrishnan V, Science. 2001 May 4;292(5518):897-902. PMID:11340196

Page seeded by OCA on Thu Mar 20 11:48:14 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1ibk.gif|left|200px]]<br /><applet load="1ibk" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ibk.gif|left|200px]]
-caption="1ibk, resolution 3.31&Aring;" />
-'''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN'''<br />
+ {{Structure
+|PDB= 1ibk |SIZE=350|CAPTION= <scene name='initialview01'>1ibk</scene>, resolution 3.31&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT IN COMPLEX WITH THE ANTIBIOTIC PAROMOMYCIN'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-IBK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=PAR:'>PAR</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IBK OCA].
+IBK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IBK OCA].
 ==Reference==
-Recognition of cognate transfer RNA by the 30S ribosomal subunit., Ogle JM, Brodersen DE, Clemons WM Jr, Tarry MJ, Carter AP, Ramakrishnan V, Science. 2001 May 4;292(5518):897-902. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11340196 11340196]
+Recognition of cognate transfer RNA by the 30S ribosomal subunit., Ogle JM, Brodersen DE, Clemons WM Jr, Tarry MJ, Carter AP, Ramakrishnan V, Science. 2001 May 4;292(5518):897-902. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11340196 11340196]
 [[Category: Protein complex]]
 [[Category: Thermus thermophilus]]
@@ Line 29: / Line 38: @@
 [[Category: ribosome]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:10:06 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:48:14 2008''