1i3v: Difference between revisions
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[[Image:1i3v.gif|left|200px]] | [[Image:1i3v.gif|left|200px]] | ||
'''THREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDED''' | {{Structure | ||
|PDB= 1i3v |SIZE=350|CAPTION= <scene name='initialview01'>1i3v</scene>, resolution 2.03Å | |||
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|LIGAND= | |||
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'''THREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDED''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1I3V is a [ | 1I3V is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3V OCA]. | ||
==Reference== | ==Reference== | ||
Lateral recognition of a dye hapten by a llama VHH domain., Spinelli S, Tegoni M, Frenken L, van Vliet C, Cambillau C, J Mol Biol. 2001 Aug 3;311(1):123-9. PMID:[http:// | Lateral recognition of a dye hapten by a llama VHH domain., Spinelli S, Tegoni M, Frenken L, van Vliet C, Cambillau C, J Mol Biol. 2001 Aug 3;311(1):123-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11469862 11469862] | ||
[[Category: Lama glama]] | [[Category: Lama glama]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: lama]] | [[Category: lama]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:45:11 2008'' | ||
Revision as of 12:45, 20 March 2008
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THREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDED
OverviewOverview
Camelids, camels and llamas, have a unique immune system able to produce heavy-chain only antibodies. Their VH domains (VHHs) are the smallest binding units produced by immune systems, and therefore suitable for biotechnological applications through heterologous expression. The recognition of protein antigens by these VHHs is rather well documented, while less is known about the VHH/hapten interactions. The recently reported X-ray structure of a VHH in complex with a copper-containing azo-dye settled the ability of VHH to recognize haptens by forming a cavity between the three complementarity-determining regions (CDR). Here we report the structures of a VHH (VHH A52) free or complexed with an azo-dye, RR1, without metal ion. The structure of the complex illustrates the involvement of CDR2, CDR3 and a framework residue in a lateral interaction with the hapten. Such a lateral combining site is comparable to that found in classical antibodies, although in the absence of the VL.
About this StructureAbout this Structure
1I3V is a Protein complex structure of sequences from Lama glama. Full crystallographic information is available from OCA.
ReferenceReference
Lateral recognition of a dye hapten by a llama VHH domain., Spinelli S, Tegoni M, Frenken L, van Vliet C, Cambillau C, J Mol Biol. 2001 Aug 3;311(1):123-9. PMID:11469862
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