1i2m: Difference between revisions
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[[Image:1i2m.jpg|left|200px]] | [[Image:1i2m.jpg|left|200px]] | ||
'''RAN-RCC1-SO4 COMPLEX''' | {{Structure | ||
|PDB= 1i2m |SIZE=350|CAPTION= <scene name='initialview01'>1i2m</scene>, resolution 1.76Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''RAN-RCC1-SO4 COMPLEX''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1I2M is a [ | 1I2M is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I2M OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1)., Renault L, Kuhlmann J, Henkel A, Wittinghofer A, Cell. 2001 Apr 20;105(2):245-55. PMID:[http:// | Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1)., Renault L, Kuhlmann J, Henkel A, Wittinghofer A, Cell. 2001 Apr 20;105(2):245-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11336674 11336674] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: g fold or gtpase fold]] | [[Category: g fold or gtpase fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:44:41 2008'' | ||
Revision as of 12:44, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
RAN-RCC1-SO4 COMPLEX
OverviewOverview
RCC1 (regulator of chromosome condensation), a beta propeller chromatin-bound protein, is the guanine nucleotide exchange factor (GEF) for the nuclear GTP binding protein Ran. We report here the 1.8 A crystal structure of a Ran*RCC1 complex in the absence of nucleotide, an intermediate in the multistep GEF reaction. In contrast to previous structures, the phosphate binding region of the nucleotide binding site is perturbed only marginally, possibly due to the presence of a polyvalent anion in the P loop. Biochemical experiments show that a sulfate ion stabilizes the Ran*RCC1 complex and inhibits dissociation by guanine nucleotides. Based on the available structural and biochemical evidence, we present a unified scenario for the GEF mechanism where interaction of the P loop lysine with an acidic residue is a crucial element for the overall reaction.
DiseaseDisease
Known diseases associated with this structure: Alveolar soft-part sarcoma OMIM:[606236], Osteolysis, familial expansile OMIM:[603499], Paget disease of bone OMIM:[603499]
About this StructureAbout this Structure
1I2M is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1)., Renault L, Kuhlmann J, Henkel A, Wittinghofer A, Cell. 2001 Apr 20;105(2):245-55. PMID:11336674
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