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[[ | ==Structure analysis of a wound-inducible lectin ipomoelin from sweet potato== | ||
<StructureSection load='4ddn' size='340' side='right' caption='[[4ddn]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ddn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ipomoea_batatas Ipomoea batatas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DDN FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r50|3r50]], [[3r51|3r51]], [[3r52|3r52]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ddn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ddn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ddn RCSB], [http://www.ebi.ac.uk/pdbsum/4ddn PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Many proteins are induced in the plant defense response to biotic stress or mechanical wounding. One group is lectins. Ipomoelin (IPO) is one of the wound-inducible proteins of sweet potato (Ipomoea batatas cv. Tainung 57) and is a Jacalin-related lectin (JRL). In this study, we resolved the crystal structures of IPO in its apo form and in complex with carbohydrates such as methyl alpha-D-mannopyranoside (Me-Man), methyl alpha-D-glucopyranoside (Me-Glc), and methyl alpha-D-galactopyranoside (Me-Gal) in different space groups. The packing diagrams indicated that IPO might represent a compact tetrameric association in the JRL family. The protomer of IPO showed a canonical beta-prism fold with 12 strands of beta-sheets but with 2 additional short beta-strands at the N terminus. A truncated IPO (DeltaN10IPO) by removing the 2 short beta-strands of the N terminus was used to reveal its role in a tetrameric association. Gel filtration chromatography confirmed IPO as a tetrameric form in solution. Isothermal titration calorimetry determined the binding constants (K(A)) of IPO and DeltaN10IPO against various carbohydrates. IPO could bind to Me-Man, Me-Glc, and Me-Gal with similar binding constants. In contrast, DeltaN10IPO showed high binding ability to Me-Man and Me-Glc but could not bind to Me-Gal. Our structural and functional analysis of IPO revealed that its compact tetrameric association and carbohydrate binding polyspecificity could be regulated by the 2 additional N-terminal beta-strands. The versatile carbohydrate binding properties of IPO might play a role in plant defense. | |||
Ipomoelin, a jacalin-related lectin with a compact tetrameric association and versatile carbohydrate binding properties regulated by its N terminus.,Chang WC, Liu KL, Hsu FC, Jeng ST, Cheng YS PLoS One. 2012;7(7):e40618. Epub 2012 Jul 11. PMID:22808208<ref>PMID:22808208</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Ipomoea batatas]] | [[Category: Ipomoea batatas]] | ||
[[Category: Chang, W C | [[Category: Chang, W C]] | ||
[[Category: Cheng, Y S | [[Category: Cheng, Y S]] | ||
[[Category: Hsu, F C | [[Category: Hsu, F C]] | ||
[[Category: Jeng, S T | [[Category: Jeng, S T]] | ||
[[Category: Liu, K L | [[Category: Liu, K L]] | ||
[[Category: Beta prism]] | [[Category: Beta prism]] | ||
[[Category: Carbohydrate binding protein]] | [[Category: Carbohydrate binding protein]] | ||
[[Category: Carbohydrate/sugar binding]] | [[Category: Carbohydrate/sugar binding]] | ||
[[Category: Sugar binding protein]] | [[Category: Sugar binding protein]] | ||