1hi5: Difference between revisions
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[[Image:1hi5.gif|left|200px]] | [[Image:1hi5.gif|left|200px]] | ||
'''EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX''' | {{Structure | ||
|PDB= 1hi5 |SIZE=350|CAPTION= <scene name='initialview01'>1hi5</scene>, resolution 1.80Å | |||
|SITE= <scene name='pdbsite=ADP:Adp+Binding+Site+For+Chain+A'>ADP</scene> | |||
|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | |||
|GENE= | |||
}} | |||
'''EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HI5 is a [ | 1HI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HI5 OCA]. | ||
==Reference== | ==Reference== | ||
Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors., Leonidas DD, Boix E, Prill R, Suzuki M, Turton R, Minson K, Swaminathan GJ, Youle RJ, Acharya KR, J Biol Chem. 2001 May 4;276(18):15009-17. Epub 2001 Jan 11. PMID:[http:// | Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors., Leonidas DD, Boix E, Prill R, Suzuki M, Turton R, Minson K, Swaminathan GJ, Youle RJ, Acharya KR, J Biol Chem. 2001 May 4;276(18):15009-17. Epub 2001 Jan 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11154698 11154698] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
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[[Category: rnase-2]] | [[Category: rnase-2]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:37:21 2008'' | ||
Revision as of 12:37, 20 March 2008
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| , resolution 1.80Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX
OverviewOverview
Eosinophil-derived neurotoxin (EDN), a basic ribonuclease found in the large specific granules of eosinophils, belongs to the pancreatic RNase A family. Although its physiological function is still unclear, it has been shown that EDN is a neurotoxin capable of inducing the Gordon phenomenon in rabbits. EDN is also a potent helminthotoxin and can mediate antiviral activity of eosinophils against isolated virions of the respiratory syncytial virus. EDN is a catalytically efficient RNase sharing similar substrate specificity with pancreatic RNase A with its ribonucleolytic activity being absolutely essential for its neurotoxic, helminthotoxic, and antiviral activities. The crystal structure of recombinant human EDN in the unliganded form has been determined previously (Mosimann, S. C., Newton, D. L., Youle, R. J., and James, M. N. G. (1996) J. Mol. Biol. 260, 540-552). We have now determined high resolution (1.8 A) crystal structures for EDN in complex with adenosine-3',5'-diphosphate (3',5'-ADP), adenosine-2',5'-di-phosphate (2',5'-ADP), adenosine-5'-diphosphate (5'-ADP) as well as for a native structure in the presence of sulfate refined at 1.6 A. The inhibition constant of these mononucleotides for EDN has been determined. The structures present the first detailed picture of differences between EDN and RNase A in substrate recognition at the ribonucleolytic active site. They also provide a starting point for the design of tight-binding inhibitors, which may be used to restrain the RNase activity of EDN.
DiseaseDisease
Known disease associated with this structure: High density lipoprotein cholesterol level QTL 7 OMIM:[131240]
About this StructureAbout this Structure
1HI5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors., Leonidas DD, Boix E, Prill R, Suzuki M, Turton R, Minson K, Swaminathan GJ, Youle RJ, Acharya KR, J Biol Chem. 2001 May 4;276(18):15009-17. Epub 2001 Jan 11. PMID:11154698
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