1gde: Difference between revisions

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Revision as of 12:21, 20 March 2008

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CRYSTAL STRUCTURE OF PYROCOCCUS PROTEIN A-1 E-FORM

OverviewOverview

We determined the crystal structure of the liganded form of alpha-aminotransferase from a hyperthermophile, Pyrococcus horikoshii. This hyperthermophilic enzyme did not show domain movement upon binding of an acidic substrate, glutamate, except for a small movement of the alpha-helix from Glu16 to Ala25. The omega-carboxyl group of the acidic substrate was recognized by Tyr70* without its side-chain movement, but not by positively charged Arg or Lys. Compared with the homologous enzymes from Thermus thermophilus HB8 and Escherichia coli, it was suggested that the more thermophilic the enzyme is, the smaller the domain movement is. This rule seems to be applicable to many other enzymes already reported.

About this StructureAbout this Structure

1GDE is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

ReferenceReference

Temperature dependence of the enzyme-substrate recognition mechanism., Ura H, Harata K, Matsui I, Kuramitsu S, J Biochem. 2001 Jan;129(1):173-8. PMID:11134972

Page seeded by OCA on Thu Mar 20 11:21:37 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1gde.jpg|left|200px]]<br /><applet load="1gde" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1gde.jpg|left|200px]]
-caption="1gde, resolution 1.8&Aring;" />
-'''CRYSTAL STRUCTURE OF PYROCOCCUS PROTEIN A-1 E-FORM'''<br />
+ {{Structure
+|PDB= 1gde |SIZE=350|CAPTION= <scene name='initialview01'>1gde</scene>, resolution 1.8&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene> and <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF PYROCOCCUS PROTEIN A-1 E-FORM'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-GDE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with <scene name='pdbligand=GLU:'>GLU</scene> and <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GDE OCA].
+GDE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GDE OCA].
 ==Reference==
-Temperature dependence of the enzyme-substrate recognition mechanism., Ura H, Harata K, Matsui I, Kuramitsu S, J Biochem. 2001 Jan;129(1):173-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11134972 11134972]
+Temperature dependence of the enzyme-substrate recognition mechanism., Ura H, Harata K, Matsui I, Kuramitsu S, J Biochem. 2001 Jan;129(1):173-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11134972 11134972]
 [[Category: Pyrococcus horikoshii]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: temperature dependence of substrate recognition]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:49:01 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:21:37 2008''