1fzr: Difference between revisions
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[[Image:1fzr.gif|left|200px]] | [[Image:1fzr.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I''' | {{Structure | ||
|PDB= 1fzr |SIZE=350|CAPTION= <scene name='initialview01'>1fzr</scene>, resolution 2.10Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Deoxyribonuclease_IV_(phage-T(4)-induced) Deoxyribonuclease IV (phage-T(4)-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.2 3.1.21.2] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1FZR is a [ | 1FZR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FZR OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I., Hadden JM, Convery MA, Declais AC, Lilley DM, Phillips SE, Nat Struct Biol. 2001 Jan;8(1):62-7. PMID:[http:// | Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I., Hadden JM, Convery MA, Declais AC, Lilley DM, Phillips SE, Nat Struct Biol. 2001 Jan;8(1):62-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11135673 11135673] | ||
[[Category: Bacteriophage t7]] | [[Category: Bacteriophage t7]] | ||
[[Category: Deoxyribonuclease IV (phage-T(4)-induced)]] | [[Category: Deoxyribonuclease IV (phage-T(4)-induced)]] | ||
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[[Category: homodimer]] | [[Category: homodimer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:16:11 2008'' | ||
Revision as of 12:16, 20 March 2008
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| , resolution 2.10Å | |||||||
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| Activity: | Deoxyribonuclease IV (phage-T(4)-induced), with EC number 3.1.21.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I
OverviewOverview
We have solved the crystal structure of the Holliday junction resolving enzyme T7 endonuclease I at 2.1 A resolution using the multiwavelength anomalous dispersion (MAD) technique. Endonuclease I exhibits strong structural specificity for four-way DNA junctions. The structure shows that it forms a symmetric homodimer arranged in two well-separated domains. Each domain, however, is composed of elements from both subunits, and amino acid side chains from both protomers contribute to the active site. While no significant structural similarity could be detected with any other junction resolving enzyme, the active site is similar to that found in several restriction endonucleases. T7 endonuclease I therefore represents the first crystal structure of a junction resolving enzyme that is a member of the nuclease superfamily of enzymes.
About this StructureAbout this Structure
1FZR is a Single protein structure of sequence from Bacteriophage t7. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I., Hadden JM, Convery MA, Declais AC, Lilley DM, Phillips SE, Nat Struct Biol. 2001 Jan;8(1):62-7. PMID:11135673
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