1fw6: Difference between revisions

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File:1fw6.gif

, resolution 2.70Å

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CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX

OverviewOverview

The MutS protein initiates DNA mismatch repair by recognizing mispaired and unpaired bases embedded in duplex DNA and activating endo- and exonucleases to remove the mismatch. Members of the MutS family also possess a conserved ATPase activity that belongs to the ATP binding cassette (ABC) superfamily. Here we report the crystal structure of a ternary complex of MutS-DNA-ADP and assays of initiation of mismatch repair in conjunction with perturbation of the composite ATPase active site by mutagenesis. These studies indicate that MutS has to bind both ATP and the mismatch DNA simultaneously in order to activate the other mismatch repair proteins. We propose that the MutS ATPase activity plays a proofreading role in DNA mismatch repair, verification of mismatch recognition, and authorization of repair.

About this StructureAbout this Structure

1FW6 is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.

ReferenceReference

Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair., Junop MS, Obmolova G, Rausch K, Hsieh P, Yang W, Mol Cell. 2001 Jan;7(1):1-12. PMID:11172706

Page seeded by OCA on Thu Mar 20 11:14:44 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1fw6.gif|left|200px]]<br /><applet load="1fw6" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1fw6.gif|left|200px]]
-caption="1fw6, resolution 2.70&Aring;" />
-'''CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX'''<br />
+ {{Structure
+|PDB= 1fw6 |SIZE=350|CAPTION= <scene name='initialview01'>1fw6</scene>, resolution 2.70&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF A TAQ MUTS-DNA-ADP TERNARY COMPLEX'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-FW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW6 OCA].
+FW6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW6 OCA].
 ==Reference==
-Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair., Junop MS, Obmolova G, Rausch K, Hsieh P, Yang W, Mol Cell. 2001 Jan;7(1):1-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11172706 11172706]
+Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair., Junop MS, Obmolova G, Rausch K, Hsieh P, Yang W, Mol Cell. 2001 Jan;7(1):1-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11172706 11172706]
 [[Category: Single protein]]
 [[Category: Thermus aquaticus]]
@@ Line 26: / Line 35: @@
 [[Category: protein-dna complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:43:25 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:14:44 2008''