1ft6: Difference between revisions
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[[Image:1ft6.jpg|left|200px]] | [[Image:1ft6.jpg|left|200px]] | ||
'''REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA''' | {{Structure | ||
|PDB= 1ft6 |SIZE=350|CAPTION= <scene name='initialview01'>1ft6</scene>, resolution 1.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=DTN:DITHIONITE'>DTN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1FT6 is a [ | 1FT6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FT6 OCA]. | ||
==Reference== | ==Reference== | ||
High-resolution structures of the oxidized and reduced states of cytochrome c554 from Nitrosomonas europaea., Iverson TM, Arciero DM, Hooper AB, Rees DC, J Biol Inorg Chem. 2001 Apr;6(4):390-7. PMID:[http:// | High-resolution structures of the oxidized and reduced states of cytochrome c554 from Nitrosomonas europaea., Iverson TM, Arciero DM, Hooper AB, Rees DC, J Biol Inorg Chem. 2001 Apr;6(4):390-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11372197 11372197] | ||
[[Category: Nitrosomonas europaea]] | [[Category: Nitrosomonas europaea]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: heme-stacking]] | [[Category: heme-stacking]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:13:44 2008'' | ||
Revision as of 12:13, 20 March 2008
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| , resolution 1.8Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA
OverviewOverview
Cytochrome c554 (cyt c554) is a tetra-heme cytochrome involved in the oxidation of NH3 by Nitrosomonas europaea. The X-ray crystal structures of both the oxidized and dithionite-reduced states of cyt c554 in a new, rhombohedral crystal form have been solved by molecular replacement, at 1.6 A and 1.8 A resolution, respectively. Upon reduction, the conformation of the polypeptide chain changes between residues 175 and 179, which are adjacent to hemes III and IV. Cyt c554 displays conserved heme-packing motifs that are present in other heme-containing proteins. Comparisons to hydroxylamine oxidoreductase, the electron donor to cyt c554, and cytochrome c nitrite reductase, an enzyme involved in nitrite ammonification, reveal substantial structural similarity in the polypeptide chain surrounding the heme core environment. The structural determinants of these heme-packing motifs extend to the buried water molecules that hydrogen bond to the histidine ligands to the heme iron. In the original structure determination of a tetragonal crystal form, a cis peptide bond between His129 and Phe130 was identified that appeared to be stabilized by crystal contacts. In the rhombohedral crystal form used in the present high-resolution structure determination, this peptide bond adopts the trans conformation, but with disallowed angles of phi and psi.
About this StructureAbout this Structure
1FT6 is a Single protein structure of sequence from Nitrosomonas europaea. Full crystallographic information is available from OCA.
ReferenceReference
High-resolution structures of the oxidized and reduced states of cytochrome c554 from Nitrosomonas europaea., Iverson TM, Arciero DM, Hooper AB, Rees DC, J Biol Inorg Chem. 2001 Apr;6(4):390-7. PMID:11372197
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