1ex3: Difference between revisions
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[[Image:1ex3.gif|left|200px]] | [[Image:1ex3.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF BOVINE CHYMOTRYPSINOGEN A (TETRAGONAL)''' | {{Structure | ||
|PDB= 1ex3 |SIZE=350|CAPTION= <scene name='initialview01'>1ex3</scene>, resolution 3.0Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF BOVINE CHYMOTRYPSINOGEN A (TETRAGONAL)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1EX3 is a [ | 1EX3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX3 OCA]. | ||
==Reference== | ==Reference== | ||
Protein crystallization by design: chymotrypsinogen without precipitants., Pjura PE, Lenhoff AM, Leonard SA, Gittis AG, J Mol Biol. 2000 Jul 7;300(2):235-9. PMID:[http:// | Protein crystallization by design: chymotrypsinogen without precipitants., Pjura PE, Lenhoff AM, Leonard SA, Gittis AG, J Mol Biol. 2000 Jul 7;300(2):235-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10873462 10873462] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Chymotrypsin]] | [[Category: Chymotrypsin]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:01:23 2008'' | ||
Revision as of 12:01, 20 March 2008
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| , resolution 3.0Å | |||||||
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| Activity: | Chymotrypsin, with EC number 3.4.21.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF BOVINE CHYMOTRYPSINOGEN A (TETRAGONAL)
OverviewOverview
Protein crystals are usually obtained by an empirical approach based on extensive screening to identify suitable crystallization conditions. In contrast, we have used a systematic predictive procedure to produce data-quality crystals of bovine chymotrypsinogen A and used them to obtain a refined X-ray structure to 3 A resolution. Measurements of the osmotic second virial coefficient of chymotrypsinogen solutions were used to identify suitable solvent conditions, following which crystals were grown for approximately 30 hours by ultracentrifugal crystallization, without the use of any precipitants. Existing structures of chymotrypsinogen were obtained in solutions including 10-30 % ethanol, whereas simple buffered NaCl solutions were used here. The protein crystallized in the tetragonal space group P4(1)2(1)2, with one molecule per asymmetric unit. The quality of the refined map was very high throughout, with the main-chain atoms of all but four residues clearly defined and with nearly all side-chains also defined. Although only minor differences are seen compared to the structures previously reported, they indicate the possibility of structural changes due to the crystallization conditions used in those studies. Our results show that more systematic crystallization of proteins is possible, and that the procedure can expand the range of conditions under which crystals can be grown successfully and can make new crystal forms available.
About this StructureAbout this Structure
1EX3 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
ReferenceReference
Protein crystallization by design: chymotrypsinogen without precipitants., Pjura PE, Lenhoff AM, Leonard SA, Gittis AG, J Mol Biol. 2000 Jul 7;300(2):235-9. PMID:10873462
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