1edy: Difference between revisions
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[[Image:1edy.gif|left|200px]] | [[Image:1edy.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF RAT ALPHA 1-MACROGLOBULIN RECEPTOR BINDING DOMAIN''' | {{Structure | ||
|PDB= 1edy |SIZE=350|CAPTION= <scene name='initialview01'>1edy</scene>, resolution 2.3Å | |||
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'''CRYSTAL STRUCTURE OF RAT ALPHA 1-MACROGLOBULIN RECEPTOR BINDING DOMAIN''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1EDY is a [ | 1EDY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EDY OCA]. | ||
==Reference== | ==Reference== | ||
Structure of a rat alpha 1-macroglobulin receptor-binding domain dimer., Xiao T, DeCamp DL, Spran SR, Protein Sci. 2000 Oct;9(10):1889-97. PMID:[http:// | Structure of a rat alpha 1-macroglobulin receptor-binding domain dimer., Xiao T, DeCamp DL, Spran SR, Protein Sci. 2000 Oct;9(10):1889-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11106161 11106161] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: pseudo-symmetric dimer]] | [[Category: pseudo-symmetric dimer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:54:18 2008'' | ||
Revision as of 11:54, 20 March 2008
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CRYSTAL STRUCTURE OF RAT ALPHA 1-MACROGLOBULIN RECEPTOR BINDING DOMAIN
OverviewOverview
Alpha-macroglobulin inhibits a broad spectrum of proteinases by forming macromolecular cages inside which proteinases are cross-linked and trapped. Upon formation of a complex with proteinase, alpha-macroglobulin undergoes a large conformational change that results in the exposure of its receptor-binding domain (RBD). Engagement of this domain by alpha-macroglobulin receptor permits clearance of the alpha-macroglobulin: proteinase complex from circulation. The crystal structure of rat alpha1-macroglobulin RBD has been determined at 2.3 A resolution. The RBD is composed of a nine-stranded beta-sandwich and a single alpha-helix that has been implicated as part of the receptor binding site and that lies on the surface of the beta-sandwich. The crystallographic asymmetric unit contains a dimer of RBDs related by approximate twofold symmetry such that the putative receptor recognition sites of the two monomers are contiguous. By gel filtration and ultracentrifugation, it is shown that RBD dimers form in solution with a dissociation constant of approximately 50 microM. The structure of the RBD dimer might mimic a conformation of transformed alpha-macroglobulin in which the proposed receptor binding residues are exposed on one face of the dimer. A pair of phenylalanine residues replaces a cystine that is conserved in other members of the macroglobulin family. These residues participate in a network of aromatic side-chain interactions that appears to stabilize the dimer interface.
About this StructureAbout this Structure
1EDY is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a rat alpha 1-macroglobulin receptor-binding domain dimer., Xiao T, DeCamp DL, Spran SR, Protein Sci. 2000 Oct;9(10):1889-97. PMID:11106161
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