1dod: Difference between revisions

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Ligands:

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THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS

OverviewOverview

Para-hydroxybenzoate hydroxylase inserts oxygen into substrates by means of the labile intermediate, flavin C(4a)-hydroperoxide. This reaction requires transient isolation of the flavin and substrate from the bulk solvent. Previous crystal structures have revealed the position of the substrate para-hydroxybenzoate during oxygenation but not how it enters the active site. In this study, enzyme structures with the flavin ring displaced relative to the protein were determined, and it was established that these or similar flavin conformations also occur in solution. Movement of the flavin appears to be essential for the translocation of substrates and products into the solvent-shielded active site during catalysis.

About this StructureAbout this Structure

1DOD is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

ReferenceReference

The mobile flavin of 4-OH benzoate hydroxylase., Gatti DL, Palfey BA, Lah MS, Entsch B, Massey V, Ballou DP, Ludwig ML, Science. 1994 Oct 7;266(5182):110-4. PMID:7939628

Page seeded by OCA on Thu Mar 20 10:41:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1dod.gif|left|200px]]<br /><applet load="1dod" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1dod.gif|left|200px]]
-caption="1dod, resolution 2.1&Aring;" />
-'''THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS'''<br />
+ {{Structure
+|PDB= 1dod |SIZE=350|CAPTION= <scene name='initialview01'>1dod</scene>, resolution 2.1&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=DOB:2,4-DIHYDROXYBENZOIC ACID'>DOB</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DOD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=DOB:'>DOB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DOD OCA].
+DOD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DOD OCA].
 ==Reference==
-The mobile flavin of 4-OH benzoate hydroxylase., Gatti DL, Palfey BA, Lah MS, Entsch B, Massey V, Ballou DP, Ludwig ML, Science. 1994 Oct 7;266(5182):110-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7939628 7939628]
+The mobile flavin of 4-OH benzoate hydroxylase., Gatti DL, Palfey BA, Lah MS, Entsch B, Massey V, Ballou DP, Ludwig ML, Science. 1994 Oct 7;266(5182):110-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7939628 7939628]
 [[Category: Pseudomonas aeruginosa]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: oxidoreductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:18:45 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:41:39 2008''