1doa: Difference between revisions

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STRUCTURE OF THE RHO FAMILY GTP-BINDING PROTEIN CDC42 IN COMPLEX WITH THE MULTIFUNCTIONAL REGULATOR RHOGDI

OverviewOverview

The RhoGDI proteins serve as key multifunctional regulators of Rho family GTP-binding proteins. The 2.6 A X-ray crystallographic structure of the Cdc42/RhoGDI complex reveals two important sites of interaction between GDI and Cdc42. First, the amino-terminal regulatory arm of the GDI binds to the switch I and II domains of Cdc42 leading to the inhibition of both GDP dissociation and GTP hydrolysis. Second, the geranylgeranyl moiety of Cdc42 inserts into a hydrophobic pocket within the immunoglobulin-like domain of the GDI molecule leading to membrane release. The structural data demonstrate how GDIs serve as negative regulators of small GTP-binding proteins and how the isoprenoid moiety is utilized in this critical regulatory interaction.

About this StructureAbout this Structure

1DOA is a Protein complex structure of sequences from Bos taurus and Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI., Hoffman GR, Nassar N, Cerione RA, Cell. 2000 Feb 4;100(3):345-56. PMID:10676816

Page seeded by OCA on Thu Mar 20 10:41:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1doa.gif|left|200px]]<br /><applet load="1doa" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1doa.gif|left|200px]]
-caption="1doa, resolution 2.60&Aring;" />
-'''STRUCTURE OF THE RHO FAMILY GTP-BINDING PROTEIN CDC42 IN COMPLEX WITH THE MULTIFUNCTIONAL REGULATOR RHOGDI'''<br />
+ {{Structure
+|PDB= 1doa |SIZE=350|CAPTION= <scene name='initialview01'>1doa</scene>, resolution 2.60&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> and <scene name='pdbligand=GER:GERAN-8-YL GERAN'>GER</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''STRUCTURE OF THE RHO FAMILY GTP-BINDING PROTEIN CDC42 IN COMPLEX WITH THE MULTIFUNCTIONAL REGULATOR RHOGDI'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DOA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GDP:'>GDP</scene> and <scene name='pdbligand=GER:'>GER</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DOA OCA].
+DOA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DOA OCA].
 ==Reference==
-Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI., Hoffman GR, Nassar N, Cerione RA, Cell. 2000 Feb 4;100(3):345-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10676816 10676816]
+Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI., Hoffman GR, Nassar N, Cerione RA, Cell. 2000 Feb 4;100(3):345-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10676816 10676816]
 [[Category: Bos taurus]]
 [[Category: Homo sapiens]]
@@ Line 25: / Line 34: @@
 [[Category: x-ray]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:18:40 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:41:37 2008''