1dcr: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1dcr.gif|left|200px]] | [[Image:1dcr.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G-A BASE PAIRS''' | {{Structure | ||
|PDB= 1dcr |SIZE=350|CAPTION= <scene name='initialview01'>1dcr</scene>, resolution 1.60Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SPM:SPERMINE'>SPM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G-A BASE PAIRS''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1DCR is a [ | 1DCR is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCR OCA]. | ||
==Reference== | ==Reference== | ||
Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:[http:// | Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10600106 10600106] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Gao, Y G.]] | [[Category: Gao, Y G.]] | ||
| Line 22: | Line 31: | ||
[[Category: deoxyribonucleic acid]] | [[Category: deoxyribonucleic acid]] | ||
[[Category: ga mismatch]] | [[Category: ga mismatch]] | ||
[[Category: tandem ga base | [[Category: tandem ga base pair]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:36:23 2008'' | ||
Revision as of 11:36, 20 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G-A BASE PAIRS
OverviewOverview
G x A mismatched base pairs are frequently found in nucleic acids. Human centromere DNA sequences contain unusual repeating motifs, e.g. , (GAATG)n x (CATTC)n found in the human chromosome. The purine-rich strand of this repeating pentamer sequence forms duplex and hairpin structures with unusual stability. The high stability of these structures is contributed by the "sheared" G x A base pairs which present a novel recognition surface for ligands and proteins. We have solved the crystal structure, by the multiple-wavelength anomalous diffraction (MAD) method of d(CCGAATGAGG) in which the centromere core sequence motif GAATG is embedded. Three crystal forms were refined to near-atomic resolution. The structures reveal the detailed conformation of tandem G x A base pairs whose unique hydrogen-bonding surface has interesting interactions with bases, hydrated magnesium ions, cobalt(III)hexaammine, spermine, and water molecules. The results are relevant in understanding the structure associated with human centromere sequence in particular and G x A base pairs in nucleic acids (including RNA, like ribozyme) in general.
About this StructureAbout this Structure
1DCR is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:10600106
Page seeded by OCA on Thu Mar 20 10:36:23 2008