1cy9: Difference between revisions
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[[Image:1cy9.gif|left|200px]] | [[Image:1cy9.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. MONOCLINIC FORM''' | {{Structure | ||
|PDB= 1cy9 |SIZE=350|CAPTION= <scene name='initialview01'>1cy9</scene>, resolution 1.80Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. MONOCLINIC FORM''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1CY9 is a [ | 1CY9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CY9 OCA]. | ||
==Reference== | ==Reference== | ||
Conformational changes in E. coli DNA topoisomerase I., Feinberg H, Lima CD, Mondragon A, Nat Struct Biol. 1999 Oct;6(10):918-22. PMID:[http:// | Conformational changes in E. coli DNA topoisomerase I., Feinberg H, Lima CD, Mondragon A, Nat Struct Biol. 1999 Oct;6(10):918-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504724 10504724] | ||
[[Category: DNA topoisomerase]] | [[Category: DNA topoisomerase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: dna topoisomerase]] | [[Category: dna topoisomerase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:30:22 2008'' | ||
Revision as of 11:30, 20 March 2008
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| , resolution 1.80Å | |||||||
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| Activity: | DNA topoisomerase, with EC number 5.99.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. MONOCLINIC FORM
OverviewOverview
DNA topoisomerases are the enzymes responsible for maintaining the topological states of DNA. In order to change the topology of DNA, topoisomerases pass one or two DNA strands through transient single or double strand breaks in the DNA phosphodiester backbone. It has been proposed that both type IA and type II enzymes change conformation dramatically during the reaction cycle in order to accomplish these transformations. In the case of Escherichia coli DNA topoisomerase I, it has been suggested that a 30 kDa fragment moves away from the rest of the protein to create an entrance into the central hole in the protein. Structures of the 30 kDa fragment reveal that indeed this fragment can change conformation significantly. The fragment is composed of two domains, and while the domains themselves remain largely unchanged, their relative arrangement can change dramatically.
About this StructureAbout this Structure
1CY9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Conformational changes in E. coli DNA topoisomerase I., Feinberg H, Lima CD, Mondragon A, Nat Struct Biol. 1999 Oct;6(10):918-22. PMID:10504724
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