1cy8: Difference between revisions
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[[Image:1cy8.gif|left|200px]] | [[Image:1cy8.gif|left|200px]] | ||
'''COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE AND 3'-THYMIDINE MONOPHOSPHATE''' | {{Structure | ||
|PDB= 1cy8 |SIZE=350|CAPTION= <scene name='initialview01'>1cy8</scene>, resolution 2.45Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=T3P:THYMIDINE-3'-PHOSPHATE'>T3P</scene> and <scene name='pdbligand=TMP:THYMIDINE-5'-PHOSPHATE'>TMP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] | |||
|GENE= | |||
}} | |||
'''COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE AND 3'-THYMIDINE MONOPHOSPHATE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1CY8 is a [ | 1CY8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CY8 OCA]. | ||
==Reference== | ==Reference== | ||
Protein-nucleotide interactions in E. coli DNA topoisomerase I., Feinberg H, Changela A, Mondragon A, Nat Struct Biol. 1999 Oct;6(10):961-8. PMID:[http:// | Protein-nucleotide interactions in E. coli DNA topoisomerase I., Feinberg H, Changela A, Mondragon A, Nat Struct Biol. 1999 Oct;6(10):961-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504732 10504732] | ||
[[Category: DNA topoisomerase]] | [[Category: DNA topoisomerase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: relaxing enzyme]] | [[Category: relaxing enzyme]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:30:21 2008'' | ||
Revision as of 11:30, 20 March 2008
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| , resolution 2.45Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | DNA topoisomerase, with EC number 5.99.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'-THYMIDINE MONOPHOSPHATE AND 3'-THYMIDINE MONOPHOSPHATE
OverviewOverview
DNA topoisomerases are the enzymes responsible for controlling and maintaining the topological states of DNA. Type IA enzymes work by transiently breaking the phosphodiester backbone of one strand to allow passage of another strand through the break. The protein has to perform complex rearrangements of the DNA, and hence it is likely that different regions of the enzyme bind DNA with different affinities. In order to identify some of the DNA binding sites in the protein, we have solved the structures of several complexes of the 67 kDa N-terminal fragment of Escherichia coli DNA topoisomerase I with mono- and trinucleotides. There are five different binding sites in the complexes, one of which is adjacent to the active site. Two other sites are in the central hole of the protein and may represent general DNA binding regions. The positions of these sites allow us to identify different DNA binding regions and to understand their possible roles in the catalytic cycle.
About this StructureAbout this Structure
1CY8 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Protein-nucleotide interactions in E. coli DNA topoisomerase I., Feinberg H, Changela A, Mondragon A, Nat Struct Biol. 1999 Oct;6(10):961-8. PMID:10504732
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