1cw0: Difference between revisions

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Revision as of 11:29, 20 March 2008

File:1cw0.gif

, resolution 2.30Å

Ligands:

and

Coordinates:

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CRYSTAL STRUCTURE ANALYSIS OF VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE IN COMPLEX WITH A DUPLEX DNA

OverviewOverview

The crystal structure of very short patch repair (Vsr) endonuclease, in complex with Mg2+ and with duplex DNA containing a TG mismatch, has been determined at 2.3 A resolution. In E. coli, the enzyme recognizes a TG mismatched base pair, generated after spontaneous deamination of methylated cytosines, and cleaves the phosphate backbone on the 5' side of the thymine. Extensive interactions between the DNA and the protein characterize a novel recognition mechanism, where three aromatic residues intercalate from the major groove into the DNA to strikingly deform the base pair stacking. With the presence of a cleaved DNA intermediate in the active center, the structure of the Vsr/DNA complex provides detailed insights into the catalytic mechanism for endonuclease activity.

About this StructureAbout this Structure

1CW0 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Recognition of a TG mismatch: the crystal structure of very short patch repair endonuclease in complex with a DNA duplex., Tsutakawa SE, Jingami H, Morikawa K, Cell. 1999 Dec 10;99(6):615-23. PMID:10612397

Page seeded by OCA on Thu Mar 20 10:29:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1cw0.gif|left|200px]]<br /><applet load="1cw0" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1cw0.gif|left|200px]]
-caption="1cw0, resolution 2.30&Aring;" />
-'''CRYSTAL STRUCTURE ANALYSIS OF VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE IN COMPLEX WITH A DUPLEX DNA'''<br />
+ {{Structure
+|PDB= 1cw0 |SIZE=350|CAPTION= <scene name='initialview01'>1cw0</scene>, resolution 2.30&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE ANALYSIS OF VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE IN COMPLEX WITH A DUPLEX DNA'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-CW0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CW0 OCA].
+CW0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CW0 OCA].
 ==Reference==
-Recognition of a TG mismatch: the crystal structure of very short patch repair endonuclease in complex with a DNA duplex., Tsutakawa SE, Jingami H, Morikawa K, Cell. 1999 Dec 10;99(6):615-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10612397 10612397]
+Recognition of a TG mismatch: the crystal structure of very short patch repair endonuclease in complex with a DNA duplex., Tsutakawa SE, Jingami H, Morikawa K, Cell. 1999 Dec 10;99(6):615-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10612397 10612397]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: zinc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:10:19 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:29:36 2008''