1cfm: Difference between revisions
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[[Image:1cfm.jpg|left|200px]] | [[Image:1cfm.jpg|left|200px]] | ||
'''CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII''' | {{Structure | ||
|PDB= 1cfm |SIZE=350|CAPTION= <scene name='initialview01'>1cfm</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1CFM is a [ | 1CFM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFM OCA]. | ||
==Reference== | ==Reference== | ||
X-ray structure of a truncated form of cytochrome f from chlamydomonas reinhardtii., Chi YI, Huang LS, Zhang Z, Fernandez-Velasco JG, Berry EA, Biochemistry. 2000 Jul 4;39(26):7689-701. PMID:[http:// | X-ray structure of a truncated form of cytochrome f from chlamydomonas reinhardtii., Chi YI, Huang LS, Zhang Z, Fernandez-Velasco JG, Berry EA, Biochemistry. 2000 Jul 4;39(26):7689-701. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10869174 10869174] | ||
[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: proton wire]] | [[Category: proton wire]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:23:47 2008'' | ||
Revision as of 11:23, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII
OverviewOverview
A truncated form of cytochrome f from Chlamydomonas reinhardtii (an important eukaryotic model organism for photosynthetic electron transfer studies) has been crystallized (space group P2(1)2(1)2(1); three molecules/asymmetric unit) and its structure determined to 2.0 A resolution by molecular replacement using the coordinates of a truncated turnip cytochrome f as a model. The structure displays the same folding and detailed features as turnip cytochrome f, including (a) an unusual heme Fe ligation by the alpha-amino group of tyrosine 1, (b) a cluster of lysine residues (proposed docking site of plastocyanin), and (c) the presence of a chain of seven water molecules bound to conserved residues and extending between the heme pocket and K58 and K66 at the lysine cluster. For this array of waters, we propose a structural role. Two cytochrome f molecules are related by a noncrystallographic symmetry operator which is a distorted proper 2-fold rotation. This may represent the dimeric relation of the monomers in situ; however, the heme orientation suggested by this model is not consistent with previous EPR measurements on oriented membranes.
About this StructureAbout this Structure
1CFM is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structure of a truncated form of cytochrome f from chlamydomonas reinhardtii., Chi YI, Huang LS, Zhang Z, Fernandez-Velasco JG, Berry EA, Biochemistry. 2000 Jul 4;39(26):7689-701. PMID:10869174
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