1cel: Difference between revisions

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Revision as of 11:23, 20 March 2008

File:1cel.jpg

, resolution 1.8Å

Ligands:

, , and

Activity:

Cellulose 1,4-beta-cellobiosidase, with EC number 3.2.1.91

Coordinates:

save as pdb, mmCIF, xml

THE THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI

OverviewOverview

Cellulose is the major polysaccharide of plants where it plays a predominantly structural role. A variety of highly specialized microorganisms have evolved to produce enzymes that either synergistically or in complexes can carry out the complete hydrolysis of cellulose. The structure of the major cellobiohydrolase, CBHI, of the potent cellulolytic fungus Trichoderma reesei has been determined and refined to 1.8 angstrom resolution. The molecule contains a 40 angstrom long active site tunnel that may account for many of the previously poorly understood macroscopic properties of the enzyme and its interaction with solid cellulose. The active site residues were identified by solving the structure of the enzyme complexed with an oligosaccharide, o-iodobenzyl-1-thio-beta-cellobioside. The three-dimensional structure is very similar to a family of bacterial beta-glucanases with the main-chain topology of the plant legume lectins.

About this StructureAbout this Structure

1CEL is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

ReferenceReference

The three-dimensional crystal structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Reinikainen T, Ruohonen L, Pettersson G, Knowles JK, Teeri TT, Jones TA, Science. 1994 Jul 22;265(5171):524-8. PMID:8036495

Page seeded by OCA on Thu Mar 20 10:23:25 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1cel.jpg|left|200px]]<br /><applet load="1cel" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1cel.jpg|left|200px]]
-caption="1cel, resolution 1.8&Aring;" />
-'''THE THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI'''<br />
+ {{Structure
+|PDB= 1cel |SIZE=350|CAPTION= <scene name='initialview01'>1cel</scene>, resolution 1.8&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=IBZ:2-IODOBENZYLTHIO GROUP'>IBZ</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91]
+|GENE=
+}}
+'''THE THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-CEL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=IBZ:'>IBZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CEL OCA].
+CEL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CEL OCA].
 ==Reference==
-The three-dimensional crystal structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Reinikainen T, Ruohonen L, Pettersson G, Knowles JK, Teeri TT, Jones TA, Science. 1994 Jul 22;265(5171):524-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8036495 8036495]
+The three-dimensional crystal structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei., Divne C, Stahlberg J, Reinikainen T, Ruohonen L, Pettersson G, Knowles JK, Teeri TT, Jones TA, Science. 1994 Jul 22;265(5171):524-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8036495 8036495]
 [[Category: Cellulose 1,4-beta-cellobiosidase]]
 [[Category: Single protein]]
@@ Line 21: / Line 30: @@
 [[Category: hydrolase(o-glycosyl)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:05:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:23:25 2008''