1c04: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1c04.gif|left|200px]] | [[Image:1c04.gif|left|200px]] | ||
'''IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI''' | {{Structure | ||
|PDB= 1c04 |SIZE=350|CAPTION= <scene name='initialview01'>1c04</scene>, resolution 5.0Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1C04 is a [ | 1C04 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C04 OCA]. | ||
==Reference== | ==Reference== | ||
Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit., Ban N, Nissen P, Hansen J, Capel M, Moore PB, Steitz TA, Nature. 1999 Aug 26;400(6747):841-7. PMID:[http:// | Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit., Ban N, Nissen P, Hansen J, Capel M, Moore PB, Steitz TA, Nature. 1999 Aug 26;400(6747):841-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10476961 10476961] | ||
[[Category: Haloarcula marismortui]] | [[Category: Haloarcula marismortui]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 21: | Line 30: | ||
[[Category: low resolution model]] | [[Category: low resolution model]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:18:09 2008'' | ||
Revision as of 11:18, 20 March 2008
| |||||||
| , resolution 5.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
IDENTIFICATION OF KNOWN PROTEIN AND RNA STRUCTURES IN A 5 A MAP OF THE LARGE RIBOSOMAL SUBUNIT FROM HALOARCULA MARISMORTUI
OverviewOverview
We have calculated at 5.0 A resolution an electron-density map of the large 50S ribosomal subunit from the bacterium Haloarcula marismortui by using phases derived from four heavy-atom derivatives, intercrystal density averaging and density-modification procedures. More than 300 base pairs of A-form RNA duplex have been fitted into this map, as have regions of non-A-form duplex, single-stranded segments and tetraloops. The long rods of RNA crisscrossing the subunit arise from the stacking of short, separate double helices, not all of which are A-form, and in many places proteins crosslink two or more of these rods. The polypeptide exit channel was marked by tungsten cluster compounds bound in one heavy-atom-derivatized crystal. We have determined the structure of the translation-factor-binding centre by fitting the crystal structures of the ribosomal proteins L6, L11 and L14, the sarcin-ricin loop RNA, and the RNA sequence that binds L11 into the electron density. We can position either elongation factor G or elongation factor Tu complexed with an aminoacylated transfer RNA and GTP onto the factor-binding centre in a manner that is consistent with results from biochemical and electron microscopy studies.
About this StructureAbout this Structure
1C04 is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.
ReferenceReference
Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit., Ban N, Nissen P, Hansen J, Capel M, Moore PB, Steitz TA, Nature. 1999 Aug 26;400(6747):841-7. PMID:10476961
Page seeded by OCA on Thu Mar 20 10:18:09 2008