1brl: Difference between revisions
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[[Image:1brl.jpg|left|200px]] | [[Image:1brl.jpg|left|200px]] | ||
'''THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION''' | {{Structure | ||
|PDB= 1brl |SIZE=350|CAPTION= <scene name='initialview01'>1brl</scene>, resolution 2.4Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] | |||
|GENE= | |||
}} | |||
'''THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1BRL is a [ | 1BRL is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. The following page contains interesting information on the relation of 1BRL with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb78_1.html Luciferase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRL OCA]. | ||
==Reference== | ==Reference== | ||
Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution., Fisher AJ, Raushel FM, Baldwin TO, Rayment I, Biochemistry. 1995 May 23;34(20):6581-6. PMID:[http:// | Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution., Fisher AJ, Raushel FM, Baldwin TO, Rayment I, Biochemistry. 1995 May 23;34(20):6581-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7756289 7756289] | ||
[[Category: Alkanal monooxygenase (FMN-linked)]] | [[Category: Alkanal monooxygenase (FMN-linked)]] | ||
[[Category: Luciferase]] | [[Category: Luciferase]] | ||
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[[Category: monooxygenase]] | [[Category: monooxygenase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:14:50 2008'' | ||
Revision as of 11:14, 20 March 2008
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| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION
OverviewOverview
Luciferases are a class of enzymes that generate light in the visible spectrum. Luciferase from luminous marine bacteria is an alpha-beta heterodimer monooxygenase that catalyzes the oxidation of FMNH2 and a long-chain aliphatic aldehyde. The X-ray crystal structure of bacterial luciferase from Vibrio harveyi has been determined to 2.4 A resolution. The structure was solved by a combination of multiple isomorphous replacement and molecular averaging between the two heterodimers in the asymmetric unit. Each subunit folds into a (beta/alpha)8 barrel motif, and dimerization is mediated through a parallel four-helix bundle centered on a pseudo 2-fold axis that relates the structurally similar subunits. The vicinity of the active site has been identified on the alpha subunit by correlations with similar protein motifs and previous biochemical studies. The structure presented here represents the first molecular model of a bioluminescent enzyme.
About this StructureAbout this Structure
1BRL is a Protein complex structure of sequences from Vibrio harveyi. The following page contains interesting information on the relation of 1BRL with [Luciferase]. Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution., Fisher AJ, Raushel FM, Baldwin TO, Rayment I, Biochemistry. 1995 May 23;34(20):6581-6. PMID:7756289
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