1b4c: Difference between revisions
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[[Image:1b4c.jpg|left|200px]] | [[Image:1b4c.jpg|left|200px]] | ||
'''SOLUTION STRUCTURE OF RAT APO-S100B USING DIPOLAR COUPLINGS''' | {{Structure | ||
|PDB= 1b4c |SIZE=350|CAPTION= <scene name='initialview01'>1b4c</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= S100BETA FROM RATTUS NORVEGICUS (RAT) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |||
}} | |||
'''SOLUTION STRUCTURE OF RAT APO-S100B USING DIPOLAR COUPLINGS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1B4C is a [ | 1B4C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B4C OCA]. | ||
==Reference== | ==Reference== | ||
The use of dipolar couplings for determining the solution structure of rat apo-S100B(betabeta)., Drohat AC, Tjandra N, Baldisseri DM, Weber DJ, Protein Sci. 1999 Apr;8(4):800-9. PMID:[http:// | The use of dipolar couplings for determining the solution structure of rat apo-S100B(betabeta)., Drohat AC, Tjandra N, Baldisseri DM, Weber DJ, Protein Sci. 1999 Apr;8(4):800-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10211826 10211826] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Weber, D J.]] | [[Category: Weber, D J.]] | ||
[[Category: calcium- binding protein]] | [[Category: calcium- binding protein]] | ||
[[Category: dipolar | [[Category: dipolar coupling]] | ||
[[Category: ef-hand]] | [[Category: ef-hand]] | ||
[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
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[[Category: three-dimensional structure]] | [[Category: three-dimensional structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:06:06 2008'' | ||
Revision as of 11:06, 20 March 2008
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| Gene: | S100BETA FROM RATTUS NORVEGICUS (RAT) (Rattus norvegicus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF RAT APO-S100B USING DIPOLAR COUPLINGS
OverviewOverview
The relative orientations of adjacent structural elements without many well-defined NOE contacts between them are typically poorly defined in NMR structures. For apo-S100B(betabeta) and the structurally homologous protein calcyclin, the solution structures determined by conventional NMR exhibited considerable differences and made it impossible to draw unambiguous conclusions regarding the Ca2+-induced conformational change required for target protein binding. The structure of rat apo-S100B(betabeta) was recalculated using a large number of constraints derived from dipolar couplings that were measured in a dilute liquid crystalline phase. The dipolar couplings orient bond vectors relative to a single-axis system, and thereby remove much of the uncertainty in NOE-based structures. The structure of apo-S100B(betabeta) indicates a minimal change in the first, pseudo-EF-hand Ca2+ binding site, but a large reorientation of helix 3 in the second, classical EF-hand upon Ca2+ binding.
About this StructureAbout this Structure
1B4C is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
The use of dipolar couplings for determining the solution structure of rat apo-S100B(betabeta)., Drohat AC, Tjandra N, Baldisseri DM, Weber DJ, Protein Sci. 1999 Apr;8(4):800-9. PMID:10211826
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