1aqh: Difference between revisions
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[[Image:1aqh.gif|left|200px]] | [[Image:1aqh.gif|left|200px]] | ||
'''ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS''' | {{Structure | ||
|PDB= 1aqh |SIZE=350|CAPTION= <scene name='initialview01'>1aqh</scene>, resolution 2.00Å | |||
|SITE= <scene name='pdbsite=AVE:Active+Site'>AVE</scene>, <scene name='pdbsite=CDE:Chloride+Is+An+Activator+For+This+Enzyme'>CDE</scene> and <scene name='pdbsite=CUM:Ca'>CUM</scene> | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] | |||
|GENE= AMY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228 Pseudoalteromonas haloplanktis]) | |||
}} | |||
'''ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1AQH is a [ | 1AQH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudoalteromonas_haloplanktis Pseudoalteromonas haloplanktis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AQH OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor., Aghajari N, Feller G, Gerday C, Haser R, Protein Sci. 1998 Mar;7(3):564-72. PMID:[http:// | Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor., Aghajari N, Feller G, Gerday C, Haser R, Protein Sci. 1998 Mar;7(3):564-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9541387 9541387] | ||
[[Category: Alpha-amylase]] | [[Category: Alpha-amylase]] | ||
[[Category: Pseudoalteromonas haloplanktis]] | [[Category: Pseudoalteromonas haloplanktis]] | ||
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[[Category: psychrophilic enzyme]] | [[Category: psychrophilic enzyme]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:01:00 2008'' | ||
Revision as of 11:01, 20 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , and | ||||||
| Ligands: | and | ||||||
| Gene: | AMY (Pseudoalteromonas haloplanktis) | ||||||
| Activity: | Alpha-amylase, with EC number 3.2.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS
OverviewOverview
Alteromonas haloplanctis is a bacterium that flourishes in Antarctic sea-water and it is considered as an extreme psychrophile. We have determined the crystal structures of the alpha-amylase (AHA) secreted by this bacterium, in its native state to 2.0 angstroms resolution as well as in complex with Tris to 1.85 angstroms resolution. The structure of AHA, which is the first experimentally determined three-dimensional structure of a psychrophilic enzyme, resembles those of other known alpha-amylases of various origins with a surprisingly greatest similarity to mammalian alpha-amylases. AHA contains a chloride ion which activates the hydrolytic cleavage of substrate alpha-1,4-glycosidic bonds. The chloride binding site is situated approximately 5 angstroms from the active site which is characterized by a triad of acid residues (Asp 174, Glu 200, Asp 264). These are all involved in firm binding of the Tris moiety. A reaction mechanism for substrate hydrolysis is proposed on the basis of the Tris inhibitor binding and the chloride activation. A trio of residues (Ser 303, His 337, Glu 19) having a striking spatial resemblance with serine-protease like catalytic triads was found approximately 22 angstroms from the active site. We found that this triad is equally present in other chloride dependent alpha-amylases, and suggest that it could be responsible for autoproteolytic events observed in solution for this cold adapted alpha-amylase.
About this StructureAbout this Structure
1AQH is a Single protein structure of sequence from Pseudoalteromonas haloplanktis. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor., Aghajari N, Feller G, Gerday C, Haser R, Protein Sci. 1998 Mar;7(3):564-72. PMID:9541387
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