1amh: Difference between revisions

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Revision as of 10:59, 20 March 2008

File:1amh.jpg

, resolution 2.5Å

Sites:

and

Ligands:

Activity:

Trypsin, with EC number 3.4.21.4

Coordinates:

save as pdb, mmCIF, xml

UNCOMPLEXED RAT TRYPSIN MUTANT WITH ASP 189 REPLACED WITH SER (D189S)

OverviewOverview

Structure-based mutational analysis of serine protease specificity has produced a large database of information useful in addressing biological function and in establishing a basis for targeted design efforts. Critical issues examined include the function of water molecules in providing strength and specificity of binding, the extent to which binding subsites are interdependent, and the roles of polypeptide chain flexibility and distal structural elements in contributing to specificity profiles. The studies also provide a foundation for exploring why specificity modification can be either straightforward or complex, depending on the particular system.

About this StructureAbout this Structure

1AMH is a Single protein structure of sequence from Rattus rattus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of substrate specificity in the serine proteases., Perona JJ, Craik CS, Protein Sci. 1995 Mar;4(3):337-60. PMID:7795518

Page seeded by OCA on Thu Mar 20 09:59:32 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1amh.jpg|left|200px]]<br /><applet load="1amh" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1amh.jpg|left|200px]]
-caption="1amh, resolution 2.5&Aring;" />
-'''UNCOMPLEXED RAT TRYPSIN MUTANT WITH ASP 189 REPLACED WITH SER (D189S)'''<br />
+ {{Structure
+|PDB= 1amh |SIZE=350|CAPTION= <scene name='initialview01'>1amh</scene>, resolution 2.5&Aring;
+|SITE= <scene name='pdbsite=CAA:Catalytic+Site'>CAA</scene> and <scene name='pdbsite=CAB:Catalytic+Site'>CAB</scene>
+|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]
+|GENE=
+}}
+'''UNCOMPLEXED RAT TRYPSIN MUTANT WITH ASP 189 REPLACED WITH SER (D189S)'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-AMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Known structural/functional Sites: <scene name='pdbsite=CAA:Catalytic+Site'>CAA</scene> and <scene name='pdbsite=CAB:Catalytic+Site'>CAB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AMH OCA].
+AMH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AMH OCA].
 ==Reference==
-Structural basis of substrate specificity in the serine proteases., Perona JJ, Craik CS, Protein Sci. 1995 Mar;4(3):337-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7795518 7795518]
+Structural basis of substrate specificity in the serine proteases., Perona JJ, Craik CS, Protein Sci. 1995 Mar;4(3):337-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7795518 7795518]
 [[Category: Rattus rattus]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: substrate specificity hydrolase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:46:07 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:59:32 2008''