P53-DNA Recognition: Difference between revisions

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Jaime Prilusky, Remo Rohs, Bailey Holmes, Ana Carolina Dantas Machado, Eran Hodis, Julia Tam, Masha Karelina, Sharon Kim, Skyler Saleebyan, Keziah Kim, Joseph M. Steinberger, Eric Martz, Alexander Berchansky, Michal Harel, Angel Herraez, Joel L. Sussman

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 ===Minor Groove Shape Readout===
-Most commonly, however, the residue Arg248 is found mutated in human tumors, <scene name='Sandbox_Reserved_170/Arg248/2'>Arg248 contacts the minor groove</scene> although it does not usually form hydrogen bonds with the bases. Arg248 was shown to recognize regions of narrow minor groove associated with enhanced negative electrostatic potential ('''Figure 7''')<ref name='kitayner'/>.  This observation provides a novel molecular explanation of the importance of Arg248 for p53-DNA binding and its role in cancer. The described mechanism known as '''shape readout''' was found to be broadly employed by arginine residues<ref name="nature">Rohs R, West SM, Sosinsky A, Liu P, Mann RS, Honig B. The role of DNA shape in protein-DNA recognition. Nature. 2009;461(7268):1248-53. [http://www.ncbi.nlm.nih.gov/pubmed/19865164 PMID:19865164].</ref>.
+Most commonly, however, the residue Arg248 is found mutated in human tumors. <scene name='Sandbox_Reserved_170/Arg248/2'>Arg248 contacts the minor groove</scene> although it does not usually form hydrogen bonds with the bases. Arg248 was shown to recognize regions of narrow minor groove associated with enhanced negative electrostatic potential ('''Figure 7''')<ref name='kitayner'/>.  This observation provides a novel molecular explanation of the importance of Arg248 for p53-DNA binding and its role in cancer. The described mechanism known as '''shape readout''' was found to be broadly employed by arginine residues<ref name="nature">Rohs R, West SM, Sosinsky A, Liu P, Mann RS, Honig B. The role of DNA shape in protein-DNA recognition. Nature. 2009;461(7268):1248-53. [http://www.ncbi.nlm.nih.gov/pubmed/19865164 PMID:19865164].</ref>.
 ==Hoogsteen vs.  Watson-Crick Base Pair in p53 Binding Sites==