1a2d: Difference between revisions
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'''PYRIDOXAMINE MODIFIED MURINE ADIPOCYTE LIPID BINDING PROTEIN''' | {{Structure | ||
|PDB= 1a2d |SIZE=350|CAPTION= <scene name='initialview01'>1a2d</scene>, resolution 2.4Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''PYRIDOXAMINE MODIFIED MURINE ADIPOCYTE LIPID BINDING PROTEIN''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1A2D is a [ | 1A2D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2D OCA]. | ||
==Reference== | ==Reference== | ||
Structural characterization of two synthetic catalysts based on adipocyte lipid-binding protein., Ory JJ, Mazhary A, Kuang H, Davies RR, Distefano MD, Banaszak LJ, Protein Eng. 1998 Apr;11(4):253-61. PMID:[http:// | Structural characterization of two synthetic catalysts based on adipocyte lipid-binding protein., Ory JJ, Mazhary A, Kuang H, Davies RR, Distefano MD, Banaszak LJ, Protein Eng. 1998 Apr;11(4):253-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9680187 9680187] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:51:53 2008'' | ||
Revision as of 10:51, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
PYRIDOXAMINE MODIFIED MURINE ADIPOCYTE LIPID BINDING PROTEIN
OverviewOverview
Adipocyte lipid-binding protein (ALBP) is a small (14.5 kDa) 10-stranded beta-barrel protein found in mammalian fat cells. The crystal structures of various holo-forms of ALBP have been solved and show the fatty acid ligand bound in a large (approximately 400 A3) cavity isolated from bulk solvent. Examination of the cavity suggests that it would be a good site for the creation of an artificial catalyst, as numerous well defined crystal structures of ALBP are available and past studies have shown the conformation to be reasonably tolerant to modification and mutagenesis. Previous work has shown ALBP to be a good protein scaffold for exploring enantio- and stereoselective reactions; two constructs, ALBP attached to either a pyridoxamine or a phenanthroline group at C117, have been chemically characterized. Both modified proteins have been crystallized and their structures solved and refined. The X-ray models have been used to examine the origin of the chiral selectivity seen in the products. It is apparent that these covalent adducts reduce the internal cavity volume, sterically limiting substrate interactions with the reactive groups, as well as solvent access to potential intermediates in the reaction pathway.
About this StructureAbout this Structure
1A2D is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structural characterization of two synthetic catalysts based on adipocyte lipid-binding protein., Ory JJ, Mazhary A, Kuang H, Davies RR, Distefano MD, Banaszak LJ, Protein Eng. 1998 Apr;11(4):253-61. PMID:9680187
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