1a1r: Difference between revisions

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Revision as of 10:51, 20 March 2008

File:1a1r.gif

, resolution 2.5Å

Sites:

, , and

Ligands:

Gene:

NS3 (GB virus C)

Coordinates:

save as pdb, mmCIF, xml

HCV NS3 PROTEASE DOMAIN:NS4A PEPTIDE COMPLEX

OverviewOverview

An estimated 1% of the global human population is infected by hepatitis C viruses (HCVs), and there are no broadly effective treatments for the debilitating progression of chronic hepatitis C. A serine protease located within the HCV NS3 protein processes the viral polyprotein at four specific sites and is considered essential for replication. Thus, it emerges as an attractive target for drug design. We report here the 2.5 angstrom resolution X-ray crystal structure of the NS3 protease domain complexed with a synthetic NS4A activator peptide. The protease has a chymotrypsin-like fold and features a tetrahedrally coordinated metal ion distal to the active site. The NS4A peptide intercalates within a beta sheet of the enzyme core.

About this StructureAbout this Structure

1A1R is a Protein complex structure of sequences from Gb virus c. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide., Kim JL, Morgenstern KA, Lin C, Fox T, Dwyer MD, Landro JA, Chambers SP, Markland W, Lepre CA, O'Malley ET, Harbeson SL, Rice CM, Murcko MA, Caron PR, Thomson JA, Cell. 1996 Oct 18;87(2):343-55. PMID:8861917

Page seeded by OCA on Thu Mar 20 09:51:42 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1a1r.gif|left|200px]]<br /><applet load="1a1r" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1a1r.gif|left|200px]]
-caption="1a1r, resolution 2.5&Aring;" />
-'''HCV NS3 PROTEASE DOMAIN:NS4A PEPTIDE COMPLEX'''<br />
+ {{Structure
+|PDB= 1a1r |SIZE=350|CAPTION= <scene name='initialview01'>1a1r</scene>, resolution 2.5&Aring;
+|SITE= <scene name='pdbsite=AC1:Active+Site,+Molecule+A'>AC1</scene>, <scene name='pdbsite=AC2:Active+Site,+Molecule+B'>AC2</scene>, <scene name='pdbsite=ZN1:Zn+Binding+Site,+Molecule+A'>ZN1</scene> and <scene name='pdbsite=ZN2:Zn+Binding+Site,+Molecule+B'>ZN2</scene>
+|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
+|ACTIVITY=
+|GENE= NS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54290 GB virus C])
+}}
+'''HCV NS3 PROTEASE DOMAIN:NS4A PEPTIDE COMPLEX'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-A1R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gb_virus_c Gb virus c] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Active+Site,+Molecule+A'>AC1</scene>, <scene name='pdbsite=AC2:Active+Site,+Molecule+B'>AC2</scene>, <scene name='pdbsite=ZN1:Zn+Binding+Site,+Molecule+A'>ZN1</scene> and <scene name='pdbsite=ZN2:Zn+Binding+Site,+Molecule+B'>ZN2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A1R OCA].
+A1R is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gb_virus_c Gb virus c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A1R OCA].
 ==Reference==
-Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide., Kim JL, Morgenstern KA, Lin C, Fox T, Dwyer MD, Landro JA, Chambers SP, Markland W, Lepre CA, O'Malley ET, Harbeson SL, Rice CM, Murcko MA, Caron PR, Thomson JA, Cell. 1996 Oct 18;87(2):343-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8861917 8861917]
+Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide., Kim JL, Morgenstern KA, Lin C, Fox T, Dwyer MD, Landro JA, Chambers SP, Markland W, Lepre CA, O'Malley ET, Harbeson SL, Rice CM, Murcko MA, Caron PR, Thomson JA, Cell. 1996 Oct 18;87(2):343-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8861917 8861917]
 [[Category: Gb virus c]]
 [[Category: Protein complex]]
@@ Line 31: / Line 40: @@
 [[Category: cofactor peptide]]
 [[Category: helicase]]
-[[Category: nonstructural proteins]]
+[[Category: nonstructural protein]]
 [[Category: serine protease]]
 [[Category: viral protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:51 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:51:42 2008''