2nvb: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
[[2nvb]] is a 4 chain structure | [[2nvb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_brockii Thermoanaerobacter brockii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NVB OCA]. | ||
==See Also== | ==See Also== | ||
Revision as of 11:22, 20 October 2012
Contribution of Pro275 to the Thermostability of the Alcohol Dehydrogenases (ADHs)Contribution of Pro275 to the Thermostability of the Alcohol Dehydrogenases (ADHs)
Analysis of the three-dimensional structures of two closely related thermophilic and hyperthermophilic alcohol dehydrogenases (ADHs) from the respective microorganisms Entamoeba histolytica (EhADH1) and Thermoanaerobacter brockii (TbADH) suggested that a unique, strategically located proline residue (Pro275) at the center of the dimerization interface might be crucial for maintaining the thermal stability of TbADH. To assess the contribution of Pro275 to the thermal stability of the ADHs, we applied site-directed mutagenesis to replace Asp275 of EhADH1 with Pro (D275P-EhADH1) and conversely Pro275 of TbADH with Asp (P275D-TbADH). The results indicate that replacing Asp275 with Pro significantly enhances the thermal stability of EhADH1 (DeltaT(1/2) </= +10 degrees C), whereas the reverse mutation in the thermophilic TbADH (P275D-TbADH) reduces the thermostability of the enzyme (DeltaT(1/2) </= -18.8 degrees C). Analysis of the crystal structures of the thermostabilized mutant D275P-EhADH1 and the thermocompromised mutant P275D-TbADH suggest that a proline residue at position 275 thermostabilized the enzymes by reducing flexibility and by reinforcing hydrophobic interactions at the dimer-dimer interface of the tetrameric ADHs. Proteins 2008. (c) 2008 Wiley-Liss, Inc.
Thermal stabilization of the protozoan Entamoeba histolytica alcohol dehydrogenase by a single proline substitution., Goihberg E, Dym O, Tel-Or S, Shimon L, Frolow F, Peretz M, Burstein Y, Proteins. 2008 Feb 7;. PMID:18260103
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this StructureAbout this Structure
2nvb is a 4 chain structure with sequence from Thermoanaerobacter brockii. Full crystallographic information is available from OCA.
See AlsoSee Also
- Alcohol dehydrogenase
- Alcohol dehydrogenase from Entamoeba histolytica
- Contribution of Pro275 to the Thermostability of the Alcohol Dehydrogenases
- D275P mutant of alcohol dehydrogenase from protozoa Entamoeba histolytica
- Tetrameric alcohol dehydrogenases
ReferenceReference
- ↑ Goihberg E, Dym O, Tel-Or S, Shimon L, Frolow F, Peretz M, Burstein Y. Thermal stabilization of the protozoan Entamoeba histolytica alcohol dehydrogenase by a single proline substitution. Proteins. 2008 Feb 7;. PMID:18260103 doi:10.1002/prot.21946