151d: Difference between revisions
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[[Image:151d.gif|left|200px]] | [[Image:151d.gif|left|200px]] | ||
'''DIVERSITY OF WATER RING SIZE AT DNA INTERFACES: HYDRATION AND DYNAMICS OF DNA-ANTHRACYCLINE COMPLEXES''' | {{Structure | ||
|PDB= 151d |SIZE=350|CAPTION= <scene name='initialview01'>151d</scene>, resolution 1.400Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=DM2:DOXORUBICIN'>DM2</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''DIVERSITY OF WATER RING SIZE AT DNA INTERFACES: HYDRATION AND DYNAMICS OF DNA-ANTHRACYCLINE COMPLEXES''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
151D is a [ | 151D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=151D OCA]. | ||
==Reference== | ==Reference== | ||
Water ring structure at DNA interfaces: hydration and dynamics of DNA-anthracycline complexes., Lipscomb LA, Peek ME, Zhou FX, Bertrand JA, VanDerveer D, Williams LD, Biochemistry. 1994 Mar 29;33(12):3649-59. PMID:[http:// | Water ring structure at DNA interfaces: hydration and dynamics of DNA-anthracycline complexes., Lipscomb LA, Peek ME, Zhou FX, Bertrand JA, VanDerveer D, Williams LD, Biochemistry. 1994 Mar 29;33(12):3649-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8142363 8142363] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Bertrand, J A.]] | [[Category: Bertrand, J A.]] | ||
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[[Category: right handed dna]] | [[Category: right handed dna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:49:39 2008'' | ||
Revision as of 10:49, 20 March 2008
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DIVERSITY OF WATER RING SIZE AT DNA INTERFACES: HYDRATION AND DYNAMICS OF DNA-ANTHRACYCLINE COMPLEXES
OverviewOverview
In crystallographic structures of biological macromolecules, one can observe many hydration rings that originate at one water molecule, pass via hydrogen bonds through several others, and return to the original water molecule. Five-membered water rings have been thought to occur with greater frequency than other ring sizes. We describe a quantitative assessment of relationships between water ring size and frequency of occurrence in the vicinity of nucleic acid interfaces. This report focuses on low-temperature X-ray crystallographic structures of two anthracyclines, adriamycin (ADRI) and daunomycin (DAUN), bound to d(CGATCG) and on several DNA structures published previously by others. We have obtained excellent low-temperature (-160 degrees C, LT) X-ray intensity data for d(CGATCG)-adriamycin and d(CGATCG)-daunomycin with a multiwire area detector. The LTX-ray data sets contain 20% (daunomycin, LT-DAUN) and 35% (adriamycin, LT-ADRI) more reflections than were used to derive the original room-temperature (15 degrees C) structures [Frederick, C.A., Williams, L.D., Ughetto, G., van der Marel, G. A., van Boom, J.H., Rich, A., & Wang, A.H.-J. (1990) Biochemistry 29, 2538-2549]. The results show that five-membered water rings are not preferred over other ring sizes. This assessment is consistent with our observation of broad dispersion W-W-W angles (sigma = 20 degrees). In addition, we report that the thermal mobility, distinct from the static disorder, of the amino sugar of daunomycin and adriamycin is significantly greater than that of the rest of the complex. This mobility implies that if the central AT base pair is switched to a CG base pair, there should be a low energy cost in avoiding the guanine amino group. The energy difference (for the sugar-binding preference) between d(CGTACG) and d(CGCGCG) could be considerably less than 20 kcal/mol, a value proposed previously from computation.
About this StructureAbout this Structure
151D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Water ring structure at DNA interfaces: hydration and dynamics of DNA-anthracycline complexes., Lipscomb LA, Peek ME, Zhou FX, Bertrand JA, VanDerveer D, Williams LD, Biochemistry. 1994 Mar 29;33(12):3649-59. PMID:8142363
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